Table 1 Strains and plasmids used in this study
NameDescriptionaReference
Plasmids
pUC-Δ42clean deletion vector for generating homozygous and heterozygous Δbgl in T. thermophilus HB27, ori pUC, AmpRthis study
pCT3FK-2allele exchange vector for generating ΔpyrE::kat and HL01, ori pBR322, KmRthis study
pJ-pyrFEintermediate vector for generating pJ-ΔpyrE::blm, ori pJET, AmpRthis study
pJ-ΔpyrE::blmallele exchange vector for generating ΔpyrE::blm and HL01, ori pJET, AmpR, BlmRthis study
pMK-parB-sgfppMK18 derived vector, allowing expression of parB-sGFP in T. thermophilus, Tth (repA), Ec (oriE), KmRLi et al. 2015
pUC-ΔparA::blmallele exchange vector for generating ΔparA::blm in T. thermophilus HB27, ori pUC, AmpR, BlmRthis study
pUC-ΔmreB::katallele exchange vector for generating ΔmreB::kat in T. thermophilus HB27, ori pUC, AmpR, KmRthis study
Strains
HB27T. thermophilus wild typeDSM 7039
HB27 wt/Δ-bglT. thermophilus HB27 heterozygous derivative with bgl clean deletedthis study
HB27 ΔpyrE::katT. thermophilus HB27 homozygous derivative with pyrE replaced by katthis study
HB27 ΔpyrE::blmT. thermophilus HB27 homozygous derivative with pyrE replaced by blmthis study
HL01T. thermophilus HB27 heterozygous derivative with pyrE replaced by kat and blm simultaneouslythis study
HB27/ParB-sGFPT. thermophilus HB27 derivative permitting expression of ParB-sGFPLi et al. 2015
ΔmreB::katparA::blmT. thermophilus HB27 derivative with parA replaced by blm and mreB replaced by katthis study
168B. subtilis wild-type strainDSM 402
  • a Ec (oriE), replication origin for E. coli; Tth (repA), replication origin for T. thermophilus.