Table 1 Schematic layout of experimental design for tank 2 in osmotic stress phenotyping. The tank comprised 144 pots arranged in a 16 row by 9 column array. Each cell in the figure represents a triplet of pots (across 3 columns within the row) and is termed a main plot. The first part of the label in each cell is the DH line that was allocated to all 3 pots within the main plot. Two of the pots within each main plot were allocated to the stress treatment and the remaining pot was allocated to the control treatment (see Supplementary Fig. S1). The numbers of tillers measured for each treatment in the main plot is given as the final part of the label (e.g., 2, 3 means there were measurements for 2 tillers from the control pot and 3 from the treated pots) Genetic clones of DH lines are shown using equality symbols (e.g., CH#135 (=151) means line CH#135 was genetically identical to line CH#151)
Columns
1-34-67-9
Rows1CH#55 (=43,56,57): 2, 3CH#49: 2, 4CH#46: 1, 3
2CH#60: 2, 3CH#57 (=43,55,57): 3, 5CH#56 (=43,55,57): 2, 3
3CH#81 (=80): 2, 2CH#74: 3, 3CH#71: 4, 6
4CH#86: 2, 4CH#83: 3, 4CH#82: 2, 4
5CH#89: 2, 2CH#88: 3, 5CH#87: 2, 3
6CH#93: 2, 3CH#91: 3, 2CH#90: 2, 4
7CH#98: 3, 2CH#97: 3, 7CH#94: 1, 2
8CH#102: 2, 2CH#101: 2, 4CH#100: 2, 3
9CH#108: 2, 4CH#107: 1, 2CH#106: 1, 4
10CH#121: 2, 4CH#119: 4, 4CH#112: 2, 3
11CH#128: 3, 5CH#127: 2, 5CH#126: 2, 5
12CH#135 (=151): 2, 2CH#134: 4, 5CH#133: 2, 4
13CH#145 (=149): 1, 1CH#141: 3, 6CH#138: 2, 4
14CH#154: 2, 5CH#147: 2, 3
15CH#165: 2, 2CH#160: 4, 3CH#155: 2, 2
16CH#169: 2, 4