Table 2 Defective attachment or cell morphology
GeneCG numberPhenotypeRNAi strain*Libraryato -Gal4en-Gal4Predicted off targets
EgfrCG10079Small CA cells that express low levels of the deiattachment-RFP marker, thinning of the cap cells close to the cap/CA attachment site107130KK+
43267GDNT
43268GD
JF01696TRiPNT+
JF01083TRiPNT
JF01084TRiPNT
JF01368TRiPNT+
cpoCG43738Small, slightly elongated CA cells that express very low levels of the deiattachment-RFP marker, thinning of the cap cells close to the cap/CA attachment site14385GD+610**
JF02996TRiP
CG13653CG13653Small CA cells that express low levels of the deiattachment-RFP marker, thinning of the cap cells close to the cap/CA attachment site15436GD+0
106259KK
fryCG32045Small, slightly elongated CA cells40309GD+633**
103569KK
edCG12676Small CA cell, occasional detachment of cap cells (mostly mild phenotypes)104279KKNT+1
3087GDNTVery mild phenotype0
938GDNT
Eb1CG3265Small, slightly elongated CA cells that express very low levels of the deiattachment-RFP marker24451GD+0
HM05093TRiP
WASpCG1520Small, slightly elongated CA cells that express very levels of the deiattachment-RFP marker13757GDNT+0
108220KKNTmild phenotype0
pyrCG13194Slightly elongated CA cells, shorter than normal cap cells, longer than normal ligament cells36524GD+
36523GD+
srCG7847Defective CA cells, detachment of cap cells, longer than normal ligament cells105282KK+Lethal
9921GD+/−
JF02781TRiP+ /-
mysCG1560Abnormal connection between the cap and CA cells (detachment of the cap cells or thinning of the cap cells in the cap/CA attachment region). Abnormally short cap cells and longer than normal ligament cells29619GD+Lethal
29620GD
103704KKVery mild phenotype
HMS00043TRiP+Lethal
JF02819TRiPNT
sensCG32120Uneven length of cap cells. Expansion of the deiChO-GFP expression into the region of the sensory unit106028KK+0
Rac 1CG2248Uneven length of cap cells. Long ligament cells49246GDNT
49247GD+1
50349GD+/−
50350GD+/−
rawCG12437Uneven length of cap cells. Expansion of the deiChO-GFP expression into the region of the sensory unit24532GD+0
101255KK
JF01382TRiPNT
  • * VDRC or BDSC transformant ID.

  • ** High number of off targets.

  • NT – not tested.

  • Table 2 lists the thirteen genes identified in the screen whose knockdown by RNAi led to defective pattern of attachment or cell morphology. The RNAi strains directed against each of the genes, the phenotype they caused, and the ability of each RNAi strain to cause a phenotype when expressed under the regulation of ato-Gal4 and en-Gal4 are listed. The number of predicted off targets is indicated for RNAi strains whose phenotypes were not reproduced by additional RNAi strains directed against the same gene.