Table 2 Plasmids
PlasmidaAntibioticMarkerDescriptionAddgene ID
pYZ033AmpRSp ura4Entry vector for S. pombe CRISPR-Cas9 system.98404
pYZ292AmpRSc LEU2Entry vector for S. pombe CRISPR-Cas9 system.102686
pYZ293AmpRSp leu1Entry vector for S. pombe CRISPR-Cas9 system.102687
pYZ294AmpRkanMXEntry vector for S. pombe CRISPR-Cas9 system.102688
pYZ300AmpRSp his3Entry vector for S. pombe CRISPR-Cas9 system.102689
pYZ301AmpRSp lys9Entry vector for S. pombe CRISPR-Cas9 system.102690
pYZ145AmpRDonor DNA for leu1 deletion in S. pombe98405
pYZ146AmpRSp ura4Cas9-gRNA plasmid for leu198406
pYZ149AmpRDonor DNA for his3 deletion in S. pombe98407
pYZ164AmpRSp ura4Cas9-gRNA plasmid for his398408
pYZ172AmpRDonor DNA for lys9 deletion in S. pombe98409
pYZ173AmpRSp ura4Cas9-gRNA plasmid for lys998410
pYZ155AmpRSp ura4nmt1 promoter: expression vector in S. pombe98411
pYZ156AmpRSp ura4nmt41 promoter: expression vector in S. pombe98412
pYZ157AmpRSp ura4nmt81 promoter: expression vector in S. pombe98413
pYZ190AmpRSp ura4General purpose vector in S. pombe98428
  • ID, identifier; AmpR, ampicillin; CRISPR, clustered regularly interspaced short palindromic repeats; gRNA, guide RNA.

  • a Only the newly designed plasmids with the Sp ura4 marker are shown here. Additional plasmids with BsaI-pad, leu1, his3, and lys9 are described in Table S2.