Table 4 Two-locus transmission ratios at hms1 and hms2 in backcross progeny of doubly heterozygous ILs
hms1;hms2a
bbNcG;GG;NN;GN;NP
IL-GG2930.310.200.240.25
IL-GN2320.280.240.250.22
IL-NG3540.300.130.300.28***
IL-NN3200.430.150.220.19****
Average0.330.180.250.24
GIL-G2980.320.050.350.28****
NIL-G4500.400.130.240.23****
GIL-N1040.340.030.300.34****
NIL-N2120.320.100.300.29***
Average0.340.080.300.28
  • * P < 0.05, ** P < 0.01, *** P < 0.005, and **** P < 0.0001 based on χ2 tests of observed frequencies vs. the Mendelian expectation. G, M. guttatus background; N, M. nasutus background; IL, introgression line.

  • a Two-locus allelic combination at hms1 and hms2 inherited from IL parent. G = M. guttatus allele; N = M. nasutus allele.

  • b Backcrosses using ILs (M. guttatus background = IL-G; M. nasutus background = IL-N) to the IM62 line of M. guttatus (G) and the SF5 line of M. nasutus (N). ♀ indicates the maternal parent and ♂ indicates the paternal parent.

  • c Number of progeny assessed.