Table 1 Genotype and allele frequencies at hms1 and hms2 in an M. nasutus-M. guttatus F2 population (N = 5487)
Allele FrequencyaGenotype Frequencyb
LocusOOE
hms10.49:0.510.22:0.55:0.23****0.24:0.50:0.26
hms20.62:0.38****0.38:0.48:0.140.38:0.47:0.14
  • **** P < 0.0001 based on χ2 tests of observed frequencies vs. the Mendelian expectation with 2 d.f. for genotypes and 1 d.f. for allele frequencies. O, observed; E, expected.

  • a O allele frequencies are reported as M. guttatus:M. nasutus (G:N). At hms2, but not hms1, allele frequencies significantly differ from the Mendelian expectation (0.5:0.5).

  • b O and expected E genotype frequencies are reported as M. guttatus homozygotes:heterozygotes:M. nasutus homozygotes (GG:GN:NN). Expected genotype frequencies shown are calculated from the random union of gametes with the observed frequencies. At hms1, genotypes differ significantly (P < 0.0001) from both the Mendelian expectation (0.25:0.5:0.25) and from the expectation given the random union of gametes with the observed allele frequencies. At hms2, genotypes differ significantly (P < 0.0001) from the Mendelian expectation but not from the expectation given the random union of gametes with the observed allele frequencies.