Table 1 Number of guide RNAs ranked by sequence score (SeqScore) and included in the TKOv3
RankClassSeqScoreNo. of Candidate GuidesNo. of gRNAs AddedCumulative gRNAs in Library
11>0.01501679679
21,2,3>0.85286,41563,83464,513
31,2,3,4>0.85307,059106965,582
41,2,30.0–0.85304,477347669,058
51,2,3,4−1.0–0.85837,136189070,948