Table 2 High CRISPR/Cas9 activity at transplanted d10 site
Gene (WormBase ID)Number of Marked F1 ProgenyNumber of Clones Lacking BanI Site (%)Number of Clones Lacking BsrBI [+BssSI] Site (%)Combined Number of Clones Lacking Restriction Sites (%)
tag-68 I (WBGene00006445)4511 (24%)10 (22)21 (47)
egl-23 IV (WBGene00001190)437 (16%)
twk-18 X (WBGene00006672)365 (14%)4 [+3] (19)12 (33)
unc-58 X (WBGene00006792)4211 (26%)11 (26)22 (52)