Table 5 Top 10 reverse vaccinology candidate list from GeneMark-predicted genes
ProteinaAA LengthWeight (kDa)SCLbAdhesincProtectivedFPKMe
0079021320Extracellular0.560.7967
0199525327Extracellular0.660.7510,356
0320563362Extracellular0.581.16307
0325548949Extracellular0.551.44138
0608546652Outer Membrane0.500.5497
0633543947Extracellular0.510.68156
0655531735Extracellular0.540.70156
0869055059Extracellular0.540.7060
0869556160Extracellular0.630.76880
088601088Extracellular0.650.66
  • AA, amino acid; SCL, subcellular localization; FPKM, fragments per kilobase of transcript per million mapped reads; NCBI, National Center for Biotechnology Information.

  • a Protein numbering is based on the NCBI Prokaryotic Genome Annotation Pipeline from the DNA accession number (CP016908) in GenBank; the nomenclature for all proteins is preceded by BCY86_.

  • b SCL is a consensus from the three standalone programs used PSORTb3.0, SOSUI, and CELLO.

  • c Adhesin is the probability of a protein being an adhesin protein and is predicted by SPAAN from the Vaxign pipeline.

  • d Protective is a score given by the program VaxiJen, which predicts the likelihood that a protein is a protective antigen.

  • e FPKM is a parameter to quantify RNA sequencing data.