Table 6 Immunological testing through western blot analysis on the top nine protein candidates
MurineBovine CowBovine Fetus
ProteinaMWT (kDa)bAnti-HisEBA+NegEBA+NegEBA+Neg
0079025+++±+
0199537++++
0320565NANANANANANA
0325555++±+±±
0608545++++±+
0633547++±±
0655537++±
0869060++±±±±
0869558++
  • MWT, molecular weight; His, histidine; EBA, epizootic bovine abortion; Neg, negative; +, clear binding of antibodies to the protein candidate; −, no presence of antibody binding; ±, weak binding of antibody to the protein candidate. NA, not analyzed.

  • a Protein numbering is based on the NCBI prokaryotic annotation pipeline from the DNA accession number (CP016908) in GenBank; the nomenclature for all proteins is preceded by BCY86_.

  • b The observed molecular weight from western blots.