Table 1 N. crassa strains used in this study
StrainGenotypeOrigin or Reference
NCN251AFungal Genetic Stock Center (FGSC) # 987 (74-OR23-1A)
CNA33aod-2, pan-2, aNargang laboratory (Chae et al. 2007b)
PL23-40aod-5, pan-2, ANargang laboratory (Descheneau et al. 2005)
AOD2-N-HA-10aod-2, pan-2, aThis study. Transformation of CNA33 with a hygromycin  resistance plasmid carrying aod-2 with triple HA tag  coding sequence inserted after start codon
Contains an ectopic copy of aod-2 with N-terminal triple HA tag. Hygromycin resistant
AOD2-C-HA-8aod-2, pan-2, aThis study. Transformation of CNA33 with a hygromycin  resistance plasmid carrying aod-2 with triple HA tag  coding sequence inserted before stop codon
Contains an ectopic copy of aod-2 with C-terminal triple HA tag. Hygromycin resistant
AOD5-N-HA-1aod-5, pan-2, AThis study. Transformation of PL23-40 with a hygromycin  resistance plasmid carrying aod-5 with triple HA tag  coding sequence inserted after start codon
Contains an ectopic copy of aod-5 with N-terminal triple HA tag. Hygromycin resistant
AOD5-C-Myc-4aod-5, pan-2, AThis study. Transformation of PL23-40 with a hygromycin  resistance plasmid carrying aod-5 with triple myc  tag coding sequence inserted before stop codon
Contains an ectopic copy of aod-5 with C-terminal triple myc tag. Hygromycin resistant
DX13aod-2, aod-5, pan-2CNA33 × PL23-40
AOD2-C-Myc AOD5-N-HAaod-2, aod-5, pan-2This study. Cotransformation of DX13 with two  hygromycin resistance plasmids. One containing aod-2  with a triple myc tag inserted before the stop codon  and one containing aod-5 with a triple HA tag inserted  after the start codon
Contains an ectopic copy of aod-5 with N-terminal triple HA tag and ectopic copy of aod-2 with  C-terminal triple myc tag
96H9Δaod-1, A hygromycin resistantFGSC # 18947
97B1Δaod-2, a hygromycin resistantFGSC # 19465
1C3Δaod-5, a hygromycin resistantFGSC # 11227