Table 1 Initial phenotypic characterization of phd mutants
StrainaVisible PhenotypesbDye-Fillingcstr-3p::gfp Expression IndexdProportion of Dauers Formed on:e
ascr#2 (nM)
0f60600
WTWTn/a0 ± 00.4 ± 0.10.7 ± 0.1
str-3p::gfpWT0.350 ± 00.5 ± 0.10.6 ± 0.1
srbc-64; srbc-66Adult egg production delayedWT0.870 ± 00.1 ± 0.00.1 ± 0.1
srg-36 srg-37n/d0.82n/dn/dn/d
oy103Inviable eggs (25°, crude pheromone)WT0.570 ± 00 ± 00.1 ± 0.0
oy104WT0.590 ± 00.1 ± 0.10.4 ± 0.1
oy105Social, eggs laid at edge of plateWT0.530.1 ± 0.00.1 ± 0.10.2 ± 0.1
oy106pDyf0.440 ± 00 ± 00.1 ± 0.0
oy107egl, slow developmentWT0.520 ± 00.2 ± 0.10.3 ± 0.1
oy108WT0.620 ± 00.1 ± 0.10.2 ± 0.1
oy109egl, slow developmentWT0.930.1 ± 0.00 ± 00 ± 0
oy113pDyf0.620 ± 00 ± 00 ± 0
oy117WT0.650 ± 00 ± 00.2 ± 0.1
oy118WT0.560 ± 00 ± 00.1 ± 0.0
oy119WT0.420 ± 00 ± 0.10.3 ± 0.1
oy120WT0.660 ± 00 ± 00 ± 0
oy125uncWT0.600 ± 00.1 ± 0.00.1 ± 0.1
oy126WT0.460 ± 00.1 ± 0.00.1 ± 0.1
oy127egl, slow developmentsDyf0.440 ± 00 ± 00.1 ± 0.0
oy129uncpDyf0.680 ± 00 ± 00 ± 0
oy131egl, slow developmentpDyf0.550 ± 00 ± 00 ± 0
oy134Asynchronous growthWT0.930 ± 00 ± 00 ± 0
oy135Adult egg production delayedWT0.710 ± 00 ± 00.1 ± 0.0
oy137Slow developmentsDyf0.740 ± 00 ± 00 ± 0
oy138uncWT0.470 ± 00 ± 00.1 ± 0.1
oy139lon, rol, thin body sizeWT0.540 ± 00 ± 00 ± 0
oy140WT0.560 ± 00 ± 00.1 ± 0.0
oy141Dyf0.950 ± 00 ± 00 ± 0
oy142sDyf0.850 ± 00 ± 00 ± 0
oy143WT0.570 ± 00.3 ± 0.10.6 ± 0.1
oy144Inviable eggs (25°, crude pheromone)WTn/d0 ± 00 ± 00.1 ± 0.0
  • ascr, ascaroside; WT, wild-type; n/a, not applicable; n/d, not done; egl, delayed egg-laying; pDyf, partially dye-fill defective; unc, uncoordinated; sDyf, strongly dye-fill defective; lon, long; rol rollers; Dyf, fully dye-fill defective.

  • a All strains, except WT, contain stably integrated copies of the str-3p::gfp fusion gene (kyIs128).

  • b Phenotypes are reported only if they are observed in > 50% of animals. Social indicates that animals aggregate.

  • c Animals were filled with DiI and amphid neurons were visualized. Criteria for classification as WT, pDyf, sDyf, and Dyf are described in Materials and Methods.

  • d str-3p::gfp expression was observed in animals grown in the presence or absence of 1 µM each ascr#2, ascr#3, and ascr#5 at 25°, and a subjective expression value with arbitrary values of 0–10 was assigned to each animal. The index presented is the ratio of expression in animals grown on pheromone, divided by the expression in control animals. n = 30 animals/condition/trial; at least two independent trials.

  • e Numbers shown are the proportion of dauers formed in the given condition from two (0 nM) or three (60 nM, 600 nM) independent experiments at 25° with 40–110 animals per assay. Two technical replicates were performed in each experiment. Errors are SEM (standard errors of the mean).

  • f Plates contained 6 µL of ethanol which was used as the diluent for ascr#2.