Table 1 Neurospora crassa G protein−coupled receptor gene families and summary of growth/developmental and chemical sensitivity/nutrition phenotypes
Fungal GPCR ClassDescription GPCR ClassNCUaN. crassa Gene(s)bSaccharomyces cerevisiae HomologcAspergillus nidulans HomologdLinear GrowtheAsexual DevelopmentfSexual DevelopmentgChemical Sensitivityh/ Nutritioni
IFungal Pheromone05758pre-2STE2gprAAHPP, P, Aj
IIFungal Pheromone00138pre-1STE3gprBP, Aj
IIICarbon Sensory06312gpr-4GPR1NoneAH
IVStm1-like00300gpr-5YPQ1AN5720C
IVStm1-like09195gpr-6RTC2AN5720C
VcAMP Receptor-Like00786gpr-1NoneAN8262AHPC
VcAMP Receptor-like04626gpr-2NoneAN8262AHP
VcAMP Receptor-like09427gpr-3NoneAN8262AHP
VIGprK-like/RGS Domain09883gpr-7NoneAN7795 (gprK)F
VIIRat growth hormone releasing factor receptor-like03253gpr-8NoneAN6680NAk
VIIImPR-Like/PAQR03238gpr-9IZH3AN4932S
VIIImPR-like/PAQR04987gpr-10IZH2AN4932F
IXMicrobial Opsin10055nop-1YRO2, MRH1, HSP30AN3361AHM
IXMicrobial Opsin01735orp-1YRO2, MRH1, HSP30AN3361AH
XLung 7TM Superfamily00182gpr-11PTM1AN0063YE
XIGPCR89/ABA GPCR00005gpr-12YHR078wNone
XIIFamily C-like06629gpr-13NoneAN8601
XIIIDUF300 superfamily/Ps GPR1106987gpr-14YKR051WAN2232NAk
XIVlPth11-like00700gpr-15NoneAN12202RPP, P
Pth11-like02903gpr-16NoneAN0178AHF
Pth11-like04106gpr-17NoneAN7774AH
Pth11-like04931gpr-18NoneAN10886NAk
Pth11-like05101gpr-19NoneAN1930NAk
Pth11-like05187gpr-20NoneAN8661NAk
Pth11-like05189gpr-21NoneAN8661
Pth11-like05307gpr-22NoneAN0751AH
Pth11-like05829gpr-23NoneAN0751RAHC,T
Pth11-like05854gpr-24NoneAN8971FL
Pth11-like06531gpr-25NoneAN5639C
Pth11-like17171gpr-26NoneAN5664NAk
Pth11-like07538gpr-27NoneAN5664F
Pth11-like16721gpr-28NoneAN2249NAk
Pth11-like07649gpr-29NoneAN8943RAHS,C
Pth11-like07769gpr-30NoneAN4452AH
Pth11-like08429gpr-31NoneNoneIAHF
Pth11-like08431gpr-32NoneAN5664A
Pth11-like08447gpr-33NoneAN5664
Pth11-like08624gpr-34NoneAN0178
Pth11-like08718gpr-35NoneAN8951
Pth11-Like09022gpr-36NoneAN5664A
Pth11-like09201gpr-37NoneAN5664
Pth11-like09796gpr-38NoneAN8328R
Pth11-like09823gpr-39NoneAN1930AHA
  • GPCR, G protein−coupled receptor; NCU, N. crassa gene number.

  • a Based on the Broad Institute’s Neurospora crassa database (http://www.broadinstitue.org/annotation/genome/neurospora/MultiHome.html).

  • b N. crassa gene names are from the literature or designated during this study.

  • c Yeast orthologs were obtained after a Blastp search of the NCBI database, using a cut-off score of e-05 or less.

  • d A. nidulans orthologs were obtained from a Blastp search of the NCBI database, using a cut-off score of e-05 or less. Only the top hit is shown.

  • e R, reduced growth rate; I, increased growth rate relative to wild type.

  • f Defects in aerial hyphae (AH) or conidial development (C).

  • g Defects in protoperithecial (PP), perithecial (P), and/or ascospore (A) development.

  • h Chemical sensitivity phenotypes are indicated based on sensitivity or resistance to sorbitol (S), cytochalasin A (C), benomyl (B), tert-butyl hydroperoxide (T), menadione (M), FK506 (F), and fludioxonil (FL).

  • i Represented by increased or decreased growth on Avicel (A) or Yeast Extract (Y) compared with wild type.

  • j Mating type dependent.

  • k Not available; see Table S1 for details.

  • l Category XIV named in this study.