Table 2 Nucleotide polymorphism estimates for GSC genes
GeneSpeciesSθπTotπSynπNon
cycA 1D. melanogaster140.00510.00460.00250.0054
D. simulans100.00350.00440.02770.0000
cycA 2D. melanogaster150.00850.00740.01570.0010
D. simulans110.00560.00610.01640.0000
mei-P26D. melanogaster260.00620.00610.01810.0000
D. simulans210.00550.00310.01100.0000
nosD. melanogaster210.00440.00450.00900.0009
D. simulans350.00970.00930.01500.0042
piwiD. melanogaster1030.00790.00740.01960.0024
D. simulans1960.02220.02040.03680.0025
pumilio 1D. melanogaster260.00400.00460.00330.0012
D. simulans1030.02020.01690.01420.0003
pumilio 2D. melanogaster100.00520.00400.00720.0000
D. simulans330.01420.01440.03460.0005
pumilio 3D. melanogaster100.00400.00460.01720.0020
D. simulans720.04000.03880.06850.0014
pumilio 4D. melanogaster130.00210.00200.00700.000
D. simulans500.00950.00890.02070.0011
stwl 1D. melanogaster210.00920.00580.00000.0001
D. simulans170.00880.00640.01190.0000
stwl 2D. melanogaster490.00510.00480.01230.0025
D. simulans430.00530.00500.00970.0033
YbD. melanogaster880.00790.00600.01290.0028
D. simulans1110.01280.01280.02590.0085
zpgD. melanogaster410.00950.01130.04130.0001
D. simulans600.01640.01480.02860.0015
  • Each amplified region of cycA, pumilio, and stwl was analyzed separately; see the section Materials and Methods and Figure S1 for locations of each amplicon. S, segregating sites; θ, nucleotide diversity; πTot, total diversity; πsyn, synonymous diversity; πnon, nonsynonymous diversity. GSC, germline stem cell.