Table 1 Enrichment of GO terms (FDR < 0.05) and associated stress responsive genes under heat and starvation in A. glycines
GO IDGO DescriptionGene NameFold Change
Up-regulated stress responsive pathways and associated genes
 Heat
  Response to stress
  GO:0006950Response to stress (BP)ATP-dependent protease1.7
  GO:0006457Protein folding (BP)Activator of HSP 90 ATPase1.4
HSP 70 (7)1.5−1.7
HSP 70 (2)18.0−24.2
HSP 40 (DnaJ)a1.5
HSP 70 (DnaK)a (4)7.3−7.7
HSP 60 (GroEL)a (3)1.7−1.9
Stress-induced-phosphoprotein1.7
Aldehyde mitochondrial-like1.6
Acyl-coa dehydrogenase1.7
Caseinolytic peptidase b protein homolog1.7
CLN3 battenin2.7
Histone partial2.0
Ras homolog gene member c2.2
Suppressor of g2 allele of skp1 homolog1.6
Metastasis suppressor protein 1 (2)1.5−1.6
 Starvation
  Biosynthesis of basic energy components
  GO:0000271Polysaccharide biosynthetic process (BP)1,4 α-glucan-branching enzyme-like (2)2.3−2.4
  GO:0016051Carbohydrate biosynthetic process (BP)6-phosphogluconate decarboxylating-like2.0
  GO:0009250Glucan biosynthetic process (BP)α-glucan-branching enzyme-like2.1
  GO:0005978Glycogen biosynthetic process (BP)CAMKK-like2.5
  GO:0033692Cellular polysaccharide biosynthetic process (BP)Glycogen synthase (2)2.1
  GO:0005976Polysaccharide metabolic process (BP)Glycogenin2.3
Insulin receptor substrate2.2
Phosphoenolpyruvate carboxykinase2.4
  Biosynthesis of endosymbiont cell wall components
  GO:0070882Cellular cell wall organization or biogenesis (BP)D-alanine-D-alanine ligasea3.5
  GO:0000270Peptidoglycan metabolic process (BP)N-acetylmuramoyl-L-alanine amidasea(2)2.4−3.5
UDP-N-acetylmuramate-alanine ligasea6.6
Peptidoglycan glycosyltransferasea6.0
  Nucleosome assembly/organization
  GO:0034728Nucleosome assembly (BP)Histone h3 (5)2.3−3.4
  GO:0000786Nucleosome organization (BP)Histone h42.4
  GO:0006334Nucleosome (CC)Histone (3)2.1−2.6
  Oxidation-reduction/NADP binding
  GO:0055114Oxidation-reduction process (MF)Cytochrome P450 (9)2.1−5.6
  GO:0016491Oxidoreductase activity (MF)Fatty acyl-reductase (6)2.9−7.2
  GO:0050661NADP binding (MF)Glucose dehydrogenase (3)2.1−4.9
  GO:0003958NADPH-hemoprotein reductase activity (CC)Laccase 13.6
  GO:0009337Sulfite reductase complex (NADPH) (CC)Short-chain dehydrogenase reductase2.1
3-oxoacyl-acp reductase (2)4.5−11.4
NADPH cytochrome P450 reductase (3)2.8−3.0
Sulfite reductase β-component (3)12.9−24.8
  Other processes
  GO:0008234Cysteine protease activity (MF)Cathepsin b (4)2.0−3.4
Cathepsin b precursor2.7
Cathepsin b-2744 (3)4.8−5.7
Down-regulated stress responsive pathways and associated genes
 Heat
  Exoskeletal components
  GO:0042302Structural constituent of cuticle (MF)Cuticle protein (2)1.6−2.1
Cuticle protein precursor (4)1.8−3. 5
RR1 cuticle protein or precursor (3)1.8
Endocuticle structural glycoprotein (2)1.6−1.8
 Starvation
  DNA replication
  GO:0006260DNA replication (BP)DNA polymerase delta catalytic subunit2.3
  GO:0006270DNA-dependent DNA replication initiation (BP)DNA primase large subunit (2)2.3−2.4
  GO:0022616DNA strand elongation (BP)DNA replication licensing factor (5)2.0−3.6
DNA topoisomerase 2-like2.0
Flap endonuclease 12.8
gag-pol polyprotein3.2
Proliferating cell nuclear antigen (2)2.5−3.0
Replication protein (2)2.0−2.2
Ribonucleoside-diphosphate reductase large chain2.9
rrm1 protein2.3
tick partial (2)2.1
Uncharacterized transposon-derived protein3.4
  Histone Methylation and Modification
  GO:0031061Negative regulation of histone methylation (BP)DNA topoisomerase 2-like2.0
  GO:0031057Negative regulation of histone modification (BP)DNA (cytosine-5)-methyltransferase 1-like2.4
  GO:0051567Histone H3-K9 methylation (BP)Lysine-specific histone demethylase 12.3
DNA primase large subunit2.3
Lysine-specific histone2.0
  Other processes
  GO:0006002Fructose 6-phosphate metabolic process (BP)6-phosphofructokinase (4)4.2−8.5
  
  GO:0000398mRNA splicing, via spliceosome (BP)RNA-binding protein cabeza2.1
  Dead box ATP-dependent RNA helicase2.4
  Heterogeneous nuclear ribonucleoprotein h22.3
  
  GO:0051082Unfolded protein binding (MF)Calreticulin2.6
HSP 40 (DnaJ) homolog (2)2.0−2.1
HSP 90 (4)2.3−3.3
j domain-containing protein cg6693-like2
t-complex protein 1 subunit gamma-like2.2
  • Condensed list of GO categories associated with genes up-regulated and down-regulated under stress. Gene lists correspond to each separate GO term and in cases in which genes were associated with multiple terms these GO terms are grouped. The number of gene duplicates is given in parentheses with fold changes relative to control conditions. GO, Gene Ontology; FDR, false discovery rate.

  • a Indicates genes coded by the aphid primary endosymbiont Buchnera aphidicola