Table 12 Summary of in silico validation of PASA updated transcript annotations
R500 and Chiifu Transcript Structure
R500 = ChiifuR500 Different from Chiifu
Comparison of RNAseq with original and updated annotationUpdated annotation correct for both50
Annotation matches genotype2
Both annotations wrong21
Uncertain41
  • Transcripts were classified as uncertain when alternative splicing or low coverage precluded definitive assignment.