Table 5 Downstream processing of Velvet-Oases and Trinity transcripts after initial assembly
Velvet-OasesTrinity
a) Initial assembly transcripts43,81639,084
b) No. of novel transcripts remained after removing blast hits to B. rapa CDS14,5405464
c) No. of novel transcripts from (b) that have B. rapa genome blast hits11,1823789
d) Number of novel transcripts from (c) remaining after chimera removal94482377
e) Number of novel transcripts from (b) that do not have a B. rapa genome blast hit33581675
f) Number of novel transcripts from (e) remaining after blasting to NCBI nr database1218
Final number of novel transcripts (d) and (f) combined10,7064052