Table 1 Average mutation rate at protospacer 1 of phytoene desaturase gene of Nicotiana benthamiana after agro-infiltration with different constructs
Construct UsedAverage Mutation (%) at Target Sites
pCas90
pTpds10
pCas9 and pTpds1 (1:1 ratio)1.8 (2/108)
pCtpds112.7 (12/94)