Table 2 Average indel percentage in T. aestivum at different protospacers with related expression cassettes
Expression CassetteIndel (%)
Protospacer 1Protospacer 2
pCinox117.9 (14/78)NA
pCinox2NA20.7 (17/82)
pCwpds122.3 (19/85)NA
pCwpds2NA18.4 (14/76)
pCinox1212.7 (12/94)11.2 (9/80)
pCpin110.2 (8/78)8.6 (7/81)
  • NA, not applicable.