Table 2 GO term and functional classification enrichment analysis using DAVID for gene families that are expanded in the Drosophila suzukii genome as compared to other Drosophila species
Annotation ClusterEnrichment ScoreCategoryaTermbCountcPGene Families: Representative Gene Symbolsd
A. Comparison to 14 Drosophila genomes
11.81GOTERM BPGO:0006071 glycerol metabolic process38.49E−03Gpdh, CG18135, Gyk
21.75GOTERM BPGO:0032268 regulation of cellular protein metabolic process93.85E−03Acp76A, PEK, Adam, mRpL11, Tollo, Spn77Bc, Su(var)205, nos, elav
GOTERM BPGO:0010605 negative regulation of macromolecule metabolic process134.11E−03WRNexo, Acp76A, mRpL11, Spn77Bc, Su(var)205, elav, mael, Hsc70-4, PEK, E(spl)m8-HLH, fkh, lolal, nos
31.58SwissProt PIRChaperone51.54E−02CG7394, CG11267, Cnx99A, Tcp-1zeta
GOTERM BPGO:0006457 protein folding62.69E−02Hsc70-4, CG11267, Cnx99A, Tcp-1zeta, FKBP59
41.42GOTERM BPGO:0051606 detection of stimulus61.26E−02Or49a, Calx, CheB42a, Galphaq, FKBP59
SwissProt PIRSensory transduction63.13E−02Or49a, Or69a, Galphaq, FKBP59, Or22a
51.37INTERPROIPR007087 zinc finger, C2H2-type132.49E−02CG6689, term, CG1647, CG16779, CG10669, Meics, CG5316, CG4360, CG3065, CG11966, CG4318
INTERPROIPR015880 zinc finger, C2H2-like132.93E−02CG6689, CG1647, CG16779, CG10669, Meics, CG5316, CG4360, CG3065, CG11966, noi, CG4318
61.22SwissProt PIRSensory transduction63.13E−02Or49a, Or69a, Galphaq, FKBP59, Or22a
GOTERM MFGO:0005549 odorant binding63.17E−02Obp83ef, Or49a, Obp51a, CheB42a, Or69a, Or22a
GOTERM BPGO:0007186 G-protein-coupled receptor protein signaling pathway104.14E−02Or49a, Tk, D2R, Or69a, Galphaq, Gr85a, mth, Pk1r, Or22a
71.2SwissProt PIRMitochondrion inner membrane51.41E−02CG7394, ATPsyn-d, ATPsyn-b, Oscp, CG9603
GOTERM CCGO:0005753 mitochondrial proton-transporting ATP synthase complex35.02E−02ATPsyn-d, ATPsyn-b, Oscp
81.14GOTERM MFGO:0004867 serine-type endopeptidase inhibitor activity44.68E−02Acp76A, CG31515, Spn77Bc, CG3604
B. Comparison to D. ananassae, D. persimilis, D. pseudoobscura, D. willistoni, D. grimshawi, D. mojavensis, and D. virulis
12.32GOTERM BPGO:0032268 regulation of cellular protein metabolic process101.56E−03Acp76A, PEK, Acp62F, Adam, mRpL11, Tollo, Spn77Bc, Spn27A, nos, elav
GOTERM BPGO:0010605 negative regulation of macromolecule metabolic process137.20E−03WRNexo, Acp76A, Acp62F, mRpL11, Spn77Bc, Spn27A, elav, Hsc70-4, PEK, E(spl)m8-HLH, fkh, lolal, nos
22.27GOTERM BPGO:0006952 defense response92.76E−03CG6168, Sr-CI, Tollo, Drs, Lectin-galC1, TotA, TotM, Spn27A, GNBP1
GOTERM BPGO:0006955 immune response93.16E−03Sr-CI, Tollo, Drs, Lectin-galC1, Rac1, TotA, TotM, Spn27A, GNBP1
31.88SwissProt PIRGlycoprotein179.71E−03Acp76A, Acp29AB, D2R, Gr59c, Mal-A2, Gr85a, TotA, Hsc70-4, PEK, scb, y, pgant3, Drs, CG32669, rt, mth, GNBP1
GOTERM CCGO:0005576 extracellular region161.88E−02Acp76A, Acp62F, Acp29AB, CheB42a, CG34049, cher, TotA, Gpb5, Spn27A, Sgs3, Tk, y, beat-Va, Drs, TotM, GNBP1
41.75GOTERM BPGO:0006071 glycerol metabolic process39.72E−03Gpdh, CG18135, Gyk
51.58GOTERM BPGO:0045087 innate immune response61.24E−02Tollo, Drs, TotA, TotM, Spn27A, GNBP1
GOTERM BPGO:0050832 defense response to fungus33.85E−02Drs, Spn27A, GNBP1
61.43SwissProt PIRChaperone52.67E−02CG7394, CG11267, Cnx99A, Tcp-1zeta
GOTERM BPGO:0006457 protein folding63.49E−02Hsc70-4, CG11267, Cnx99A, Tcp-1zeta, FKBP59
71.42SwissProt PIRCalcium81.08E−02CG4733, CG4662, pgant3, CG17271, Cad74A, sunz, Tctp
GOTERM MFGO:0005509 calcium ion binding102.82E−02CG4733, CG4662, pgant3, Cnx99A, CG17271, Cad74A, CG42255, sunz, Tctp
81.28GOTERM BPGO:0051606 detection of stimulus61.67E−02Or49a, Calx, CheB42a, Galphaq, FKBP59
GOTERM BPGO:0009628 response to abiotic stimulus83.30E−02shep, Calx, Galphaq, TotA, TotM, mth, FKBP59
91.21INTERPROIPR007087 zinc finger, C2H2-type134.40E−02CG6689, term, CG1647, CG16779, CG10669, Meics, CG5316, CG4360, CG3065, CG11966, CG4318
INTERPROIPR015880 zinc finger, C2H2-like135.12E−02CG6689, CG1647, CG16779, CG10669, Meics, CG5316, CG4360, CG3065, CG11966, noi, CG4318
101.2SwissProt PIRMitochondrion inner membrane52.45E−02CG7394, ATPsyn-d, ATPsyn-b, Oscp, CG9603
GOTERM BPGO:0055085 transmembrane transport56.29E−02CG7394, ATPsyn-d, ATPsyn-b, CG32669, Oscp
111.16GOTERM CCGO:0005886 plasma membrane205.15E−02D2R, Tollo, Gr59c, Or69a, Gr85a, Cnx99A, Rac1, Cad74A, Oscp, Pk1r, Mical, Syn1, Sr-CI, scb, Or49a, Calx, Galphaq, mth, FKBP59, GNBP1
GOTERM BPGO:0007186 G-protein-coupled receptor protein signaling pathway105.98E−02Or49a, Tk, D2R, Gr59c, Or69a, Galphaq, Gr85a, mth, Pk1r
121.45GOTERM MFGO:0043169 cation binding445.96E−02CG6689, term, CHORD, CG9715, CG10669, CG4769, CG10916, CG5316, MICAL-like, Irk2, Cnx99A, CG17271, Cad74A, Mical, sunz, CG3065, CG11966, mud, CG4318, CG4662, CG31019, pgant3, CG33552, CG32669, Tctp, CG1647, CG16779, Meics, CG4733, x16, Mal-A2, CG4360, alph, noi, Mcm2, CD98hc, Arc1, CG42255, CG5292, nos, SF1
C. Comparison to the Melanogaster subgroupe
11.84GOTERM BPGO:0006071 glycerol metabolic process37.90E−03Gpdh, CG18135, Gyk
21.49GOTERM BPGO:0010605 negative regulation of macromolecule metabolic process133.03E−03WRNexo, mRpL11, Spn77Bc, Su(var)205, aub, elav, mael, Hsc70-4, PEK, E(spl)m8-HLH, fkh, lolal, nos
GOTERM BPGO:0032268 regulation of cellular protein metabolic process93.07E−03PEK, Adam, mRpL11, Tollo, Spn77Bc, aub, Su(var)205, nos, elav
31.38INTERPROIPR013087 zinc finger, C2H2-type/integrase, DNA-binding73.95E−02CG11966, CG6689, CG16779, CG10669, Meics, CG4360, CG3065
INTERPROIPR012934 zinc finger, AD-type54.08E−02CG6689, CG1647, CG10669, Meics, CG4318
41.34GOTERM BPGO:0007314 oocyte anterior/posterior axis specification52.30E−02mael, Tm1, lkb1, aub, nos
GOTERM BPGO:0007316 pole plasm RNA localization42.53E−02mael, Tm1, lkb1, aub
51.21GOTERM MFGO:0043169 cation binding415.03E−02CG6689, term, CHORD, CG9715, CG10669, CG4769, CG10916, CG5316, LpR1, Irk2, CG17271, Cad74A, Mical, CG3065, CG11966, CG4318, CG4662, CG31019, pgant3, CG33552, Tctp, Tim13, CG1647, CG16779, Meics, CG4733, x16, Mal-A2, CG4360, alph, noi, Mcm2, CG6767, CD98hc, Arc1, CG42255, CG5292, nos, SF1
  • BP, biological process; PIR, protein information resources; MF, molecular function; CC, cellular component.

  • a Categories used in DAVID analysis include GO subontologies for BP, MF, and CC, as well as key words from SwissProt PIR and protein domains from the INTERPRO database.

  • b Only representative terms with highly significant P values are shown for each annotation cluster. See Table S7, Table S8, Table S9, Table S10, Table S11, and Table S12 for complete lists.

  • c Count represents the number of gene families.

  • d Gene symbol for representative, most highly annotated member of each gene family is shown. All annotations shown here are from D. melanogaster.

  • e D. simulans, D. sechellia, D. melanogaster, D. yakuba, and D. erecta.