Table 2 Mutation rate based on mutation accumulation over ~170 generations
Functional DomainRelevant GenotypeSingle-Base Pair SubstitutionsInsertions or DeletionsMutation Rate OverallaFold Induction WTb
Genomic WTMSH2104.8 × 10−101
Nullmsh2Δ71407.1 × 10−08215
Structural integritymsh2-A618V81095.7 × 10−08171
msh2-R657G61417.1 × 10−08215
msh2-L183P71437.2 × 10−08220
msh2-C195Yc151588.4 × 10−08253
msh2-C345F161809.5 × 10−08287
msh2-D621Gc121447.5 × 10−08228
msh2-P640T101256.5 × 10−08198
DNA bindingmsh2-R542L41356.7 × 10−08203
msh2-D524Y141518.0 × 10−08242
ATPasemsh2-G688D151397.4 × 10−08225
msh2-G693R91467.5 × 10−08227
msh2-S695Pc141598.4 × 10−08253
msh2-S742F91568.0 × 10−08242
msh2-T743K51477.3 × 10−08223
msh2-G770R71477.4 × 10−08225
  • a Mutations per base pair per generation.

  • b Fold induction compared with a previously published rate 3.3 × 10−10 (Lynch et al. 2008).

  • c Plasmid rearrangement, effectively a null.