Table 2 Comparison of [GT/CA]19 mutagenesis results from ex vivo and in vitro assays
Ex vivo assay
MMR HeterodimerLCL721LCL1261HCT116+chr3HCT116In vitro Assay
MutS α++++a
β++
MutL α++
γ+++
Expansion MF4.4 × 10−67.2 × 10−38.0 × 10−44.8 × 10−35.6 × 10−3
Deletion MF1.7 × 10−6<1.3 × 10−44.9 × 10−37.3 × 10−31.2 × 10−2
Expansion:Deletion2.6:1>55:11:61:1.51:2
  • MMR, mismatch repair; MF, #FUdRr + Cmr colonies/Cmr colonies. Expansion MF and Deletion MF were calculated as [MF X (number of expansion or deletion mutants/total number of mutants)].

  • a Components of the MutSα mismatch recognition complex are present in HCT116 cells but cannot function in MMR because the absence of MLH1 renders the cells devoid of any MutL complex.