Table 2 Properties and phenotypes of rpb2 decreased readthrough (white) strains
GrowthaRelative Reporter Expression With Terminator From:Homology
RegionaSequence
IdentityaStructural
HomologyaDomaina
Mutation30°16°38°MPAADH2bNonebCYC1aSNR13a
A75Twtwtwtwt<wtwtwtHDPUProtrusion
Y149Dwtwtwt<<<ND><UProtrusion
D568GPSExternal 2
K191Mwtwt<<<<<<wt<<SProtrusion
I343TcLobe
N221Kwtwtwtwt<wtwtwtHDPSTSLobe
E371GHDPTSLobe
S235P<<<<<<<ND<<<TSLobe
S480PHDPSETSFork
N610SSExternal 2
D304Vwt<<<<<<<<<<HDPSSLobe
E368KPTSLobe
L566PHDPSExternal 2
V305IwtNDwt<<ND<<SLobe
F376SPTSLobe
F581SHDPSExternal 2
D407Gwtwtwtwt<NDwtwtHDPTSProtrusion
E468GFork
K625NSExternal 2
I343Twtwtwt<<<<wt<<<<<Lobe
L361P<wt<<<<<<<wt<<<<HDPSSLobe
E368Gwt<wt<<<<<<<<<<<PSLobe
I502THDPTSFork
E368Kwt<<wt<<<<<wt<<<<<PSLobe
K418M<<<<wt<wt<<<TSProtrusion
S489PTSFork
Q481Rdwtwt<<<<<<<<<<<HDPESTSFork
K537Ewtwt<<wt<<ND<<<DHDPSTSFork
  • ND, not determined; wt, wild type.

  • a Same as in Table 1.

  • b The relative intensity of blue color was scored as follows: <, light blue relative to the blue color observed for the control; <<, remaining white at the end of the assay.

  • c Strains with the single mutations in bold italics were shown to have phenotypes with the rp51-ADH2p(A)-lacZ reporter (Table 2, 3, or 4).

  • d Two independent isolates.