Table 1 Bacterial strains and plasmids
Strain or PlasmidGenotype or PhenotypeReference or Source
E. coli DH5αsupE44 ΔlacU169 (Ø80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi1 relA1Hanahan (1983)
E. coli BL21 DE3hsdS gal(λcIts857 ind1 sam7 nin5lac UV5 T7 gene 1Studier and Moffatt (1986)
E. coli EC100D pir+116F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFR)Epicentre Biotechnologies, USA
E. coli HB101F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-Boyer and Roulland-Dussoix (1969)
E. coli S17-1thi pro hsdR hsdM recA RP4 2-Tc::Mu-Knr::Tn7(Tpr Spr Smr)Simon et al. (1983)
Sphingobium fuliginis ATCC 27551Wild type strain, Smr, Pmr, OPH+Kawahara et al. (2010)
Acinetobacter sp.DS002Cmr, Smr, Ben+Unpublished results from our lab
pRSET-AAmpr, expression vectorInvitrogen, USA
pBluescript KS(II)Ampr, lacZ+, cloning vectorFermentas, USA
pET-23b(+)Ampr, expression vectorNovagen, USA
pMMB206Cmr, a low copy number broad host range expression vectorMorales et al. (1991)
pJQ210Gmr, sacB+Quandt and Hynes (1993)
pKD46Ampr, Red recombinase expression plasmidDatsenko and Wanner (2000)
pRK2013Kmr, ColE1-based restricted-host-range helper plasmidDitta et al. (1980)
pSM3Ampr, Tcr, opd gene replaced with opd::tet in plasmid pSM2Siddavattam et al. (2003)
pPDL2opd+, 37.3Kb indigenous plasmidMulbry et al. (1986)
pPDL2-Kopd+, Kmr, pPDL2 having single minitransposon EZ-Tn5<R6Kγori/KAN-2> insertionThis study
pPDL2-KTopd+, Kmr, Tetr, pPDL2-K with replacement of opd with opd::tetThis study
pP4IoriT sequence of pPDL2 cloned in pBluescript KS(II)This study
pSFT1repB gene cloned in pRSET-A as BamHI and XhoI fragmentThis study
pSFT2Tcr, Mini replicon generated by ligating oriV and repA region of pPDL2 to opd::tet cassetteThis study
pSDP7Ampr, temperature sensitive cloning vectorThis study
pSDP8Ampr, attB sequence cloned in pSDP7 as HindIII fragmentThis study
pSDP9ligA and ligB genes cloned in pET-23b(+) as NdeI and HindIII fragmentThis study