Table 2 Expression levels and RNAi knockdown efficiency as measured by quantitative RT-PCR
GeneExpression Level in the Ovary (CT value ± SD)a,bRNAi LinecPercentage Knockdown in Whole Fliesd
Positive candidates
 baz25.7 ± 0.350 (L–M)v291441***
 bbg27.4 ± 0.435 (L–M)v1597589**
 CG649826.0 ± 0.640 (L–M)v3510053*
 CG650926.3 ± 0.868 (L–M)v2249677**
Negative candidates
 CG4370734.5 ± 0.885 (L)v2584640 (ns)
 CG4395540.1 ± 1.51 (NE)v10326778**
 CG958824.3 ± 0.318 (M–H)HM0501382***
 cnk25.5 ± 0.451 (L–M)HMS0023867***
 dlg125.4 ± 0.575 (L–M)HMS0152152**
 dysc28.6 ± 0.237 (L–M)v2327832*
 Grip28.4 ± 0.671 (L–M)v10355190***
 PICK130.4 ± 0.252 (L)JF0119921**
 RHOGAP100F31.9 ± 0.874 (L)HMS0074014 (ns)
 scrib25.8 ± 0.512 (L–M)v10541250**
 sif31.2 ± 0.0700 (L)v10683216 (ns)
 Syn131.2 ± 0.500 (L)JF0265432 (ns)
 vari25.7 ± 0.0985 (L–M)HM0508772***
 X11L26.0 ± 0.463 (L–M)v2865255**
  • a Mean CT value and SD are calculated from three independent qPCR experiments. Expression summary: CT < 20, very high (VH); CT = 20–25, moderate to high (M–H); CT = 25–30, low to moderate (L–M); CT = 30–35, low (L); CT = 35–40, none to low (N–L); CT > 40, not expressed (NE).

  • b For reference, the endogenous expression levels (mean CT value ± SD) of rp49 and tub84b were 17.7 ± 0.112 and 19.1 ± 0.16, respectively.

  • c RNAi lines are from the Vienna Drosophila RNAi Center (prefixed with v) and from the Harvard Transgenic RNAi Project (prefixed with HM, HMS, or JF).

  • d Extent of reduction of target gene expression compared with gfp dsRNA control, calculated using the ΔΔCT method with rp49 expression as reference. The one-tailed unpaired t-test was used to test for significance (ns, P > 0.05; *P = 0.01–0.05; **P = 0.001–0.01; ***P < 0.001).