Table 1 Features of mwh mosaicism
Treatment and/or GenotypeaWing,
Nmwh
Clone,
nmwh Clone Frequency,
n/NSize Class (I-VIII)b and the Number of mwh Cells per CloneAverage Clone Sizec
(mwh Cell per Clone),
mFrequency of Clone Induction,
f
I.
1II.
2III.
3−4IV.
5−8V.
9−16VI.
17−32VII.
33−64VIII.
65−128
Control1081641.5963922520001.7 ± 1.01.7 × 10−4
Su-var(2)10340561.427177410001.9 ± 1.01.8 × 10−4
Su(var)3-9ptn32521.628139200001.8 ± 0.91.9 × 10−4
X-rays; 1000 Rad (150 kV, 0.5 mm Al; 500 Rad/min)121199.9**3731191594222.6 ± 1.517.2 × 10−4
X-rays; mwh+ VK16; 1000 Rad (150 kV, 0.5 mm Al; 500 Rad/min)4070.2**511000001.4 ± 0.90.2 × 10−4
Colchicine (1 μg/mL in the food. From 84-92 hAEL on)46315.8**815161167002.8 ± 1.529.4 × 10−4
EMS (25 mM for 4 hr at 84−92 hAEL)18573.2*24169242002.2 ± 1.44.6 × 10−4
Formaldehyded (0.05M in the food. from 84−92 hAEL on)461362.9**923112100001.4 ± 0.72.8 × 10−4
ldsHor-D/lds+14735.2**38267200001.7 ± 1.05.9 × 10−4
nub-Gal4; UAS-ldsHor-D; lds+/lds+2424710.3**9964453162002.2 ± 1.215.1 × 10−4
ldshor-rvP2l/Df(3R)ED5218241646.8**8443231130001.9 ± 1.08.7 × 10−4
  • * and ** indicate significantly different from the control at P < 0.05 and P < 0.01, respectively. hAEL, hours after egg laying.

  • a All the males carried the w1118-labeled X, the mwh+Y chromosome, and were homozygous for mwh. Su-var(2)103 is a dominant suppressor mutation of position-effect-variegation and Su(var)3-9ptn is an exceptionally strong position-effect-variegation enhancer mutation (Schotta et al. 2003; Ebert et al. 2004).

  • b The minimum number of cell divisions (I−VIII) required—after the loss of the mwh+Y chromosome—for the formation of clones composed from 1, 2, 3−4, etc., mwh cells. It was assumed that only one of the daughter cells becomes mwh-labeled after the loss of the mwh+Y chromosome during mitosis.

  • c Calculated from the average size class by making use of the linear relationship between size classes (I−VIII) and the log average clone size within the different size classes.

  • d 0.05M formaldehyde mixed into the food allows 50% of the larvae develop to adult (Szabad et al. 1983).