Table 6  Construction of heterothallic haploid strains with auxotrophic markers for S. mikatae, S. kudriavzevii and S. bayanus
SpeciesStrainOriginalGenotypeReference
S. mikataeJRY9171IFO 1815TMATa hoΔ::KanMX ura3Δ::HygMXThis study
S. mikataeJRY9172IFO 1815TMATα hoΔ::KanMX ura3Δ::HygMXThis study
S. mikataeJRY9173IFO 1815TMATa hoΔ::NatMX ura3Δ::HygMXThis study
S. mikataeJRY9174IFO 1815TMATα hoΔ::NatMX ura3Δ::HygMXThis study
S. mikataeJRY9175IFO 1815TMATa hoΔ::KanMX his3Δ::HygMXThis study
S. mikataeJRY9176IFO 1815TMATα hoΔ::NatMX trp1Δ::HygMXThis study
S. mikataeJRY9177IFO 1815TMATa hoΔ::KanMX his3Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9178IFO 1815TMATα hoΔ::KanMX his3Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9179IFO 1815TMATa hoΔ::NatMX his3Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9180IFO 1815TMATα hoΔ::NatMX his3Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9181IFO 1815TMATa hoΔ::KanMX trp1Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9182IFO 1815TMATα hoΔ::KanMX trp1Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9183IFO 1815TMATa hoΔ::NatMX trp1Δ::HygMX ura3Δ::HygMXThis study
S. mikataeJRY9184IFO 1815TMATα hoΔ::NatMX trp1Δ::HygMX ura3Δ::HygMXThis study
S. kudriavzeviiFM1097IFO 1802TMATα hoΔ::natMXHittinger et al. 2010
S. kudriavzeviiFM1098IFO 1802TMATa hoΔ::natMXHittinger et al. 2010
S. kudriavzeviiFM1363IFO 1802TMATα hoΔ::kanMXThis study
S. kudriavzeviiFM1403IFO 1802TMATa/MATα hoΔ::kanMX/hoΔ::kanMXThis study
S. kudriavzeviiFM1122IFO 1802TMATα hoΔ::natMX ura3Δ0This study
S. kudriavzeviiFM1141IFO 1802TMATα hoΔ::natMX ura3Δ0 trp1Δ::ScerURA3+This study
S. kudriavzeviiFM1388IFO 1802TMATα hoΔ::natMX ura3Δ0 his3Δ0This study
S. kudriavzeviiJRY9185IFO 1802TMATa hoΔ::natMX ura3Δ0This study
S. kudriavzeviiJRY9186IFO 1802TMATα hoΔ::natMX trp1Δ0This study
S. kudriavzeviiJRY9187IFO 1802TMATa hoΔ::natMX trp1Δ0 ura3Δ0This study
S. kudriavzeviiJRY9188IFO 1802TMATα hoΔ::natMX trp1Δ0 ura3Δ0This study
S. kudriavzeviiFM1109ZP 591MATa hoΔ::kanMXHittinger et al. 2010
S. kudriavzeviiFM1110ZP 591MATα hoΔ::kanMXHittinger et al. 2010
S. kudriavzeviiFM1071ZP 591MATa/MATαHittinger et al. 2010
S. kudriavzeviiFM1158ZP 591MATa/MATαThis study
S. kudriavzeviiFM1400ZP 591MATa/MATα hoΔ::kanMX/hoΔ::kanMXThis study
S. kudriavzeviiFM1340ZP 591MATa hoΔ::natMX ura3Δ0Hittinger et al. 2010
S. kudriavzeviiFM1123ZP 591MATa hoΔ::kanMX ura3Δ0Hittinger et al. 2010
S. kudriavzeviiFM1192ZP 591MATα hoΔ::kanMX ura3Δ0This study
S. kudriavzeviiFM1194ZP 591MATa hoΔ::kanMX trp1Δ0This study
S. kudriavzeviiFM1131ZP 591MATα hoΔ::kanMX trp1Δ0Hittinger et al. 2010
S. kudriavzeviiFM1183ZP 591MATa hoΔ::kanMX ura3Δ0 trp1Δ0Hittinger et al. 2010
S. kudriavzeviiFM1193ZP 591MATα hoΔ::kanMX ura3Δ0 trp1Δ0This study
S. kudriavzeviiFM1389ZP 591MATa hoΔ::kanMX ura3Δ0 his3Δ0This study
S. bayanusJRY9189CBS 7001MATa hoΔ::NatMXThis study
S. bayanusJRY9190CBS 7001MATα hoΔ::NatMXThis study
S. bayanusJRY8149CBS 7001MATa hoΔ::NatMX his3 lys2 ura3Gallagher et al. 2009
S. bayanusJRY8150CBS 7001MATα hoΔ::NatMX his3 lys2 ura3Gallagher et al. 2009
S. bayanusJRY8153CBS 7001MATa hoΔ::NatMX his3 lys2 trp ura3Gallagher et al. 2009
S. bayanusJRY8154CBS 7001MATα hoΔ::NatMX his3 lys2 trp ura3Gallagher et al. 2009
S. bayanusJRY8147CBS 7001MATa hoΔ::NatMX ade2 his3 lys2 ura3Gallagher et al. 2009
S. bayanusJRY8148CBS 7001MATα hoΔ::NatMX ade2 his3 lys2 ura3Gallagher et al. 2009
S. bayanusJRY9191CBS 7001MATa hoΔ::NatMX his3 ura3This study
S. bayanusJRY9040CBS 7001MATa hoΔ::NatMX lys2 ura3Zill et al. 2010
S. bayanusJRY9192CBS 7001MATa hoΔ::NatMX ade2 ura3This study
S. bayanusJRY9193CBS 7001MATα hoΔ::NatMX ade2 ura3This study
S. bayanusJRY9194CBS 7001MATa hoΔ::loxP his3 lys2 ura3This study
S. bayanusJRY9195CBS 7001MATα hoΔ::loxP his3 lys2 ura3This study
  • All strains are available upon request from C. T. Hittinger.