The coupling of transgenes to heat shock promoters is a widely applied method for regulating gene expression. In Caenorhabditis elegans gene induction can be controlled temporally through timing of heat shock, and spatially via targeted rescue in heat shock mutants. Here we present a method for evoking gene expression in arbitrary cells, with single-cell resolution. We use a focused pulsed infrared laser to locally induce a heat shock response in specific cells. Our method builds on and extends a previously reported method using a continuous-wave laser. In our technique the pulsed laser illumination enables a much higher degree of spatial selectivity due to diffusion of heat between pulses. We apply our method to induce transient and long-term transgene expression in embryonic, larval, and adult cells. Our method allows highly selective spatiotemporal control of transgene expression and is a powerful tool for model organism biology.
- Received July 12, 2013.
- Accepted August 16, 2013.
- Copyright © 2013 Author et al.
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