Frequency of coverage for Velvet assembly with hash length 31. The first peak in the bimodal distribution (“1”) represents the murine contigs that had lower coverage due to the larger genome size. The smaller peak (“2”) represents the frequency of assembled contigs originating from P. abortibovis. These contigs were collected for further assembly with the average coverage parameter set to 50.
↵a Amplicon size from primer G3F to G3R1 is 844 bp, and the amplicon size from G3F to G3R2 is 601 bp.
Table 2Assembly statistics for contiguous sequences at each stage of genome assembly
Unassigned bp (N)
Contigs, number of contiguous sequences; Total bp, total number of base pairs assembled; N50, contig length when the sum of contigs is >50% of total assembly; Unassigned bp (N), number of unassigned base pairs; Seq, sequencing.
Table 3Number of predicted proteins, gene length, and percent expression in predictions from the RAST gene prediction program
# of Predicted Genes
Average Gene Length (bp)
% Expressed (FPKM > 0)
Nonexpressed Hypo thetical proteins
RAST, Rapid Annotation using Subsystem Technology; FPKM, fragments per kilobase of transcript per million mapped reads.
Table 4Summary statistics of gene prediction programs
MWT, molecular weight; His, histidine; EBA, epizootic bovine abortion; Neg, negative; +, clear binding of antibodies to the protein candidate; −, no presence of antibody binding; ±, weak binding of antibody to the protein candidate. NA, not analyzed.
↵a Protein numbering is based on the NCBI prokaryotic annotation pipeline from the DNA accession number (CP016908) in GenBank; the nomenclature for all proteins is preceded by BCY86_.
↵b The observed molecular weight from western blots.