Skip to main content
  • Facebook
  • Twitter
  • YouTube
  • LinkedIn
  • Google Plus
  • Other GSA Resources
    • Genetics Society of America
    • Genetics
    • Genes to Genomes: The GSA Blog
    • GSA Conferences
    • GeneticsCareers.org
  • Log in
G3: Genes | Genomes | Genetics

Main menu

  • HOME
  • ISSUES
    • Current Issue
    • Early Online
    • Archive
  • ABOUT
    • About the journal
    • Why publish with us?
    • Editorial board
    • Contact us
  • SERIES
    • Genetics of Immunity
    • Genetics of Sex
    • Genomic Selection
    • Multiparental Populations
  • ARTICLE TYPES
    • About Article Types
    • Genome Reports
    • Meeting Reports
    • Mutant Screen Reports
    • Software and Data Resources
  • PUBLISH & REVIEW
    • Scope & publication policies
    • Submission & review process
    • Article types
    • Prepare your manuscript
    • Submit your manuscript
    • After acceptance
    • Guidelines for reviewers
  • SUBSCRIBE
    • Email alerts
    • RSS feeds
  • Other GSA Resources
    • Genetics Society of America
    • Genetics
    • Genes to Genomes: The GSA Blog
    • GSA Conferences
    • GeneticsCareers.org

User menu

Search

  • Advanced search
G3: Genes | Genomes | Genetics

Advanced Search

  • HOME
  • ISSUES
    • Current Issue
    • Early Online
    • Archive
  • ABOUT
    • About the journal
    • Why publish with us?
    • Editorial board
    • Contact us
  • SERIES
    • Genetics of Immunity
    • Genetics of Sex
    • Genomic Selection
    • Multiparental Populations
  • ARTICLE TYPES
    • About Article Types
    • Genome Reports
    • Meeting Reports
    • Mutant Screen Reports
    • Software and Data Resources
  • PUBLISH & REVIEW
    • Scope & publication policies
    • Submission & review process
    • Article types
    • Prepare your manuscript
    • Submit your manuscript
    • After acceptance
    • Guidelines for reviewers
  • SUBSCRIBE
    • Email alerts
    • RSS feeds
Previous ArticleNext Article

Quantitative Trait Locus and Haplotype Analyses of Wild and Crop-Mimic Traits in U.S. Weedy Rice

Muhamad S. Mispan, Lihua Zhang, Jiuhuan Feng and Xing-You Gu
G3: Genes, Genomes, Genetics June 1, 2013 vol. 3 no. 6 1049-1059; https://doi.org/10.1534/g3.113.006395
Muhamad S. Mispan
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lihua Zhang
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiuhuan Feng
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xing-You Gu
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Xingyou.gu@sdstate.edu
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
Loading

Abstract

Conspecific weeds retained characteristics from wild ancestors and also developed crop mimicries for adaptation and competitiveness. This research was conducted to identify quantitative trait loci (QTL) associated with the wild and crop-mimic traits and to determine haplotype variants for QTL-rich regions in U.S. weedy rice. An F2 population from the cross between a cultivated (EM93-1) and a U.S. weedy (US1) rice line was evaluated for six wild and eight crop-mimic traits in a greenhouse to identify the QTL. A core collection of 27 U.S. weedy red rice lines and 14 AA-genome wild rice lines were determined for the haplotype variants. A total of 49 QTL were identified, with 45 collocated as clusters on 14 genomic segments. The number of haplotypes across the 14 segments was lower in the weedy (6.1 ± 2.4) than in the wild (7.5 ± 1.8) rice sample. Both samples shared ~50% haplotypes (wild-like). The EM93-1−like haplotypes accounted for a greater proportion (30 ± 26%) of the haplotypes in the weedy than in the wild (7 ± 10%) rice. Based on haplotype patterns for the 14 QTL cluster regions, 26 of the 28 red rice lines were clustered into two groups corresponding to the black-hull awned and straw-hull awnless morphological types, respectively. The QTL analysis demonstrated that conspecific weed-crop differentiation involved many genomic segments with multiple loci regulating natural variation for adaptation and competitiveness. The haplotype analysis revealed that U.S. weedy rice retained large blocks of linkage disequilibrium for the multiple loci from the wild relatives and also incorporated haplotypes from cultivars.

  • weedy rice
  • adaptation
  • crop mimicry
  • quantitative trait locus
  • haplotype

Weeds are unwanted plants that have adapted to human-disturbed environments and compete with crops for limited natural resources (Harlan 1965; Baker 1974; Booth et al. 2003). Many crop species, such as barley (Hordeum vulgare), oat (Avena sativa), rice (Oryza sativa), sorghum (Sorghum bicolor), and wheat (Triticum aestivum), have conspecific or congeneric weeds (Ellstrand et al. 1999). Crops and their conspecific weeds share evolutionary origins and produce fertile hybrids resulting in gene flow from cultivars to the local weed populations. Conspecific weeds retained characteristics from wild relatives (e.g., seed shattering and dormancy) and also developed crop mimicries (e.g., plant/seedling morphologies and rapid vegetative growth) to enhance the adaptation and competitiveness in agro-ecosystems. Knowledge of specific genes regulating natural variation for the wild and crop mimic traits is essential to understand weed evolution and adaptation and to develop weed management and transgene mitigation strategies.

Weedy rice refers to various forms of unwanted plants that belong to the Oryza genus and are phenotypically intermediate between cultivated and wild (Oryza spp.) rice (Oka 1988; Delouche et al. 2007). Asian cultivated rice (O. sativa) originated from the wild ancestors (O. rufipogon and O. nivara) at multiple sites in South to Southeast Asia and differentiated into the indica and japonica subspecies, which are cultivated mainly in tropical and temperate areas, respectively (Kush and Brar 2002). Based on diagnostic characteristics for the subspecific and the wild-cultivated variation, weedy rice could be classified into indica- and japonica-like groups, with each consisting of wild-like and crop-mimic subgroups (Tang and Morishima 1997; Suh et al. 1997). Red rice (i.e., red or brown pericarp-colored weedy rice) prevails in both indica- and japonica-like weed populations. The indica-like weed populations in Tropical Asia, where there was/is wild rice, could originate from natural hybridization between cultivated and wild rice or from natural variants of wild rice (Oka 1988). The japonica-like and some indica-like weed populations in the areas historically absent of wild rice (e.g., Europe and the United States) may originate from old cultivars adaptive to the local environmental conditions or natural hybridization between indica and japonica cultivars (Tang and Morishima 1997; Suh et al. 1997; Ishikawa et al. 2005; Cao et al. 2006; Londo and Schaal 2007). U.S. weedy rice was likely derived from contaminants in imported seeds of commercial rice (Delouche et al. 2007) and has population structures closest to the indica and aus ecotypes of the indica subspecies (Reagon et al. 2010).

Quantitative trait locus (QTL) analysis, map-based cloning, and gene-based haplotype analysis have been used to identify genes and their genomic organization or haplotype patterns for wild and crop mimic traits differentiated between weedy and cultivated rice. A total of 26 QTL were identified for 16 traits from a cross between a japonica cultivar and a japonica-like weedy rice line from France (Bres-Patry et al. 2001) and 20 QTL identified for five wild traits from a cross between an indica cultivar and a Thailand indica-like weedy rice line (Gu et al. 2005a; Ye et al. 2010). Many of the reported QTL were collocated on a small number of chromosomal segments. Some collocated QTL (weed adaptive haplotypes), such as qSD7-1/qPC7 or qSD4/qHC4 for seed dormancy and pericarp or hull color, could not be broken across several generations (Gu et al. 2005b). The qSD7-1/qPC7 QTL were map-based cloned as a single gene (Gu et al. 2011), which is the red pericarp gene Rc encoding a bHLH family transcription factor (Sweeney et al. 2006; Furukawa et al. 2007). The functional Rc alleles in weedy red rice were clustered into two groups corresponding to the indica and japonica subspecies and the Rc alleles in U.S. red rice originated more likely from indica landraces than from wild rice (Reagon et al. 2010; Gu et al. 2011). Haplotype analysis for the seed shattering genes qsh1 (Konishi et al. 2006) and sh4 (Li et al. 2006) suggested that these loci did not contribute to the trait variation in U.S. weedy rice populations (Thurber et al. 2010). Similarly, the haplotype diversity for the semi-dwarf1 (sd1) region was not associated with the phenotypic variation for plant growth-related traits (Reagon et al. 2011).

Despite the relatively short history of rice cultivation in the United States, weedy rice populations in the country have differentiated into many ecotypes and structures. Collected ecotypes are often classified based on seed morphologies (e.g., pericarp and hull colors, awn presence/absence and grain types) and vary for multiple wild and crop mimic traits (Noldin et al. 1999; Delouche et al. 2007). The population structures were defined by genomic patterns for randomly selected short-DNA sequences (Reagon et al. 2010). There is still a considerable lack of knowledge on genes and genome structures underlying the trait variation and ecotype differentiation in weedy rice. Distinguishing from neutrally evolving DNA sequences, QTL for adaptive traits are targets of natural selections. Therefore, the objectives of this research were to identify QTL for wild and crop mimic traits, including those enhancing the competitiveness during the vegetative growth phase, and to determine haplotype patterns for the QTL-containing regions in U.S. weedy rice.

Materials and Methods

Plant materials

An F2 population from the ‘EM93-1’/‘US1’ cross was developed for QTL mapping. EM93-1 is a semi-dwarf line of indica-type cultivated rice and carries the mutant allele at sd1 (Ye et al. 2013). US1 is a pure line of indica-like weedy rice from the United States and was used in the reported research (Tang and Morishima 1997, Suh et al. 1997). A core collection of 27 U.S. weedy rice lines and 14 lines of the AA-genome wild rice (O. rufipogon, O. nivara, and O. glumipatula), which were introduced from the National Small Grains Collection, the United States Department of Agriculture–Agriculture Research Service, were used to identify haplotype variants for mapped QTL regions. All the 28 (include the parent US1) weedy rice lines are red rice and represent four morphological types: black hull awned (BHA), furrow hull awned (FHA), straw hull awned (SHA), and straw hull awnless (SH). These weedy rice lines and some wild rice lines were planted in a greenhouse to confirm the seed morphologies (see Supporting Information, Table S1, Figure S1).

Plant cultivation

Fully after-ripened (dormancy-released) seeds from the F1 and parental plants were germinated in a 30° incubator for 5 d and newly germinated seeds planted into 200-cell Plug trays filled with the rice nutrient solution (Yoshida et al. 1976) for 4 weeks to synchronize seedling size. About 500 F2 seedlings were transplanted into pots (12 × 12 × 15 cm dimensions), with one plant per pot. The pots were filled with clay soil mixed with greenhouse medium (Sunshine Growth Mix) and placed in water-tight containers (60 cm ×120 cm) in a greenhouse. The greenhouse temperatures were set at 30/23° for day/night and the day length was natural, except for the period from 35 to 70 d after germination when a short-day (10 hr) treatment was applied to induce floral initiation. Flowering time was recorded as the date when the first panicle in a plant emerged from the leaf sheath. Seeds were harvested at 40 d after flowering, air-dried in the greenhouse for 3 d before stored in a freezer (-20°) to maintain the dormancy status.

Phenotypic identification for wild and crop-mimic traits

The 14 traits were measured with one or more parameters as described in Table 1. Methods used to measure the wild traits—seed shattering (SH), seed dormancy (SD), awn (AN), and hull color (HC)—were similar to those previously described (Gu et al. 2005a). Three reproductive tillers cut from a plant were gently shaken in a bucket for 10 sec to collect shattered seeds, and shattered and nonshattered seeds were counted to estimates a shattering rate for the plant. A sample of ~30 seeds in a 9-cm Petri dish lined with a filter paper and wetted with 5 mL of water was germinated at 30° and 100% relative humidity in dark for 7 d. The mean germination (%) of three samples was used to estimate the degree of seed dormancy for a plant. AN was quantified by the mean awn length and percentage of seeds with awn for a sample of ~50 seeds from a plant. HC was measured with the ChromaMeter Minolta CR310, which transfers reflectance spectra into the L*, a*, and b* readings to quantify blackness, redness, and yellowness, respectively. L* ranges from 0 to 100, with 0 and 100 indicating completely nonreflective (black) and perfectly reflective (white), respectively. a* varies from −100 to 100, with negative and positive values indicating degrees of green and red pigmentations, respectively; a high, positive a* value indicates a high intensity of redness. b* varies from −100 to 100, with the negative and positive values indicating degrees of blue and yellow pigmentations, respectively; a high, positive b* value indicates a high intensity of yellow pigmentation. The pigment measurement was conducted using >100 seeds in a 6-cm Petri dish on a dark background and repeated three times.

View this table:
  • View inline
  • View popup
Table 1 List of wild and crop-mimic traits evaluated for the F2 EM93-1/US1 population

The crop-mimic traits include plant height (PHR)- or tiller number (TNR)-increasing rates and seed setting percentage (SSP). Plant height and the number of tillers per plant were observed at 4, 6, and 8 wk after germination, and PHR and TNR were estimated as ratios of the observations between week 6 and week 4 or week 8 and week 6. SSP was evaluated as the proportion of the number of fertilized seeds to the total number of spikelets on the three panicles used to measure the shattering rate.

Marker genotyping and linkage map construction

Genomic DNA was extracted from fresh leaf segments of the parental and F2 seedlings. Approximately 320 rice microsatellite markers (McCouch et al. 2002) were screened for polymorphism between the parents. Polymorphic markers were used to genotype a subpopulation of 188 F2 plants to develop a framework linkage map to scan for QTL. The remaining F2s were genotyped only with the markers nearest to QTL peak positions to confirm their effects. DNA extraction, marker amplification by polymerase chain reaction, and marker display by electrophoresis with nondenatured polyacrylamide gel were performed using the previously described methods (Gu et al. 2004a). The linkage map was constructed using MAPMAKER/EXP 3.0 (Lincoln et al. 1992). The marker genotyping data were also analyzed for segregation distortion by a chi-square test.

QTL mapping

The initial QTL analysis was conducted based on the subpopulation. The composite interval mapping program of the WinQTLCart software (Wang et al. 2006) was used to generate likelihood ratio distributions for individual traits to infer QTL positions and to estimate QTL additive (a) and dominance (d) effects. The QTL threshold was established by 1000 random permutations at a genome-wide type I error of 5%. The degree of dominance for a QTL was estimated by the d/a ratio. A QTL confidence interval was expressed by the length of 1-LOD (or 4.61 likelihood ratio) support region in centimorgan derived from Kosambi’s map function. Two or more QTL with overlapping 1-LOD support intervals were defined as a QTL cluster.

The QTL detected by composite interval mapping in the subpopulation were corroborated by single marker analysis (SMA) for data from the full population of ~480 F2 plants. The SMA was conducted using one-way analysis of variance with the marker nearest to a QTL peak as the indicator variable. The variables were coded as 0, 1, and 2 for the EM93-1-like homozygote, heterozygote, and US1-like homozygote, respectively, for the analysis. Analysis of variance was performed using the SAS GLM procedure (SAS Institute 2011).

Haplotype analysis

Haplotype analysis was conducted for the QTL cluster regions detected from the mapping population. The analysis was limited to the weedy and wild rice lines (Table S1) and did not include cultivars because some wild characteristics in weeds, such as a high degree of seed shattering and dormancy, black hull color, and long awn (Figure S1), are absent or very rare in cultivars. The 27 weedy and 14 wild rice lines were genotyped with all markers mapped on each of the genomic regions that encompass 1-LOD support intervals of the clustered QTL. The marker genotypes (nonallelic combinations) were used to determine haplotype variants for individual QTL cluster regions. The physical length of a haplotype was estimated based on marker positions on the Nipponbare genome sequence (Gramene 2012). The weedy and wild rice samples were compared for the number of haplotypes using a paired Student’s t-test. The marker genotyping data were subjected to a cluster analysis using the single linkage method (SAS Institute 2011) to group the U.S. weedy red rice lines and to infer phylogenetic relations among the weedy and wild rice lines.

Results

Phenotypic variation and correlation

The parental lines US1 and EM93-1 were divergent in phenotype for the six wild traits, which varied in segregation pattern in the F2 population. Both leaf sheath (LSC) and pericarp (PC) colors were scored as qualitative traits; the F2 plants could be grouped into the two (purple and green) types for LSC or three (red, brown, and white) types for PC (Figure 1, A and B). The purple or brown type was US1-like, whereas the green or white type was EM93-1-like for LSC or PC. The red type of the F2s resembled the hybrid F1s for PC, presumably resulting from the complementation of non-allelic genes assembled from the two parents. Both SH and SD are quantitative traits and their F2 frequency distributions approximated to normal distributions (Figure 1, C and D). The HC and AN traits appeared to be of both qualitative and quantitative natures. For HC, the F2 population consisted of black, brown, furrow, golden, and straw hull−colored plants and some plants had the hull tissue with multiple colors. However, using the component reflectance spectrum for blackness (L*) or yellowness (b*), the F2s could be divided into basically two groups, although variation occurred within each group (Figure 1E). For AN, the F2 population consisted of ~86% awned and ~14% awnless plants. However, the awned plants varied in awn length from ~0.5 to 5 cm or percentage of awned seeds from ~2 to 100%, and there was a nonlinear correlation between these two quantitative measurements (Figure 1F).

Figure 1
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1

Frequency distributions of wild (A−F) and crop-mimic (G−L) traits in the F2 EM93-1/US1 population. Arrows indicate the parental means.

The parent US1 had later flowering time (FT), less reproductive tillers/plant (RTN), and taller plant height (PH) than the parent EM93-1 and was similar to EM93-1 in seed numbers/plant (SN), seed weight (SW), and seed setting percentage (SSP). In the F2 population, FT, PH, RTN, SN, and SW displayed approximately normal frequency distributions and transgressive segregation (Figure 1, G−K), and only SSP had a negatively skewed frequency distribution (Figure 1L).

Phenotypic variation for the plant vegetative growth-related traits (i.e., plant height and tiller number) was observed between the two parental lines and among the F2 individuals. The range of the observed variations increased with times from week 4 to week 8 in the F2 population (Figure 2, A and D). Both PHR and TNR exhibited approximately normal frequency distributions for each of the two (weeks 4−6 and weeks 6−8) periods (Figure 2, B and C, E and F).

Figure 2
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2

Dynamic patterns of plant vegetative growth in the F2 EM93-1/US1 population. (A, D) Plant height and the number of tillers per plant at weeks 4, 6, and 8. (B−C, E−F) Frequency distributions of the plant height- and tiller number-increasing rates for week 6/week 4 (w6/w4) or w8/w6. Arrows indicate the parental means.

Each of the 14 traits measured was correlated with three (HC) to 10 (AN) of the remaining 13 traits. The correlation occurred between wild traits, crop-mimic traits, and wild and crop-mimic traits (Table 2). For example, SD was correlated with the other five wild traits and also with the two crop-mimic traits FT and SW; and the two vegetative growth-related traits PHR and TNR were negatively correlated (r = -0.21). The phenotypic correlations suggest that none of the adaptive traits was inherited independently.

View this table:
  • View inline
  • View popup
Table 2 Summary of correlation coefficients (r) between adaptive traits segregating in the F2 EM93-1/US1 population

The framework linkage map

Of the 320 markers screened, 173 are polymorphic between EM93-1 and US1, with the polymorphic rate being 54%. The subpopulation of 188 F2 plants was genotyped with 123 polymorphic markers and the genotyping data (no missing data) were used to construct a framework linkage map (Figure 3). The total length of the map was 1423 cM, with the average inter-marker distance being 13 (±7) cM. Segregation distortion was detected for markers on seven segments of chromosomes 1 and 7 to 12. Four and three of the seven segments had allelic frequencies in favor of EM93-1- and US1-derived alleles, respectively (Figure 3, see Table S2).

Figure 3
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3

Framework linkage map and QTL distribution. The map was constructed based on 188 plants from the F2 population. Vertical bars represent 12 chromosomes marked with rice microsatellite loci. Map distance in centiMorgan was derived from Kosambi’s map function. Chromosomal segments with markers displaying a segregation distortion are depicted by gray-colored or striped bars, which indicate the distortion in favor of the alleles from the parental line EM93-1 or US1. The marker in bold indicates its segregation ratio deviated most from the Mendelian expectation (Table S2). The lengths of black bars right to each chromosome indicate 1-LOD support regions for the named QTL associated with wild and crop mimic traits. The empty triangles point to the peak position of the QTL in the chromosome. QTL with overlapping 1-LOD support regions are grouped as a QTL cluster (CL). Underlined markers were used to determine haplotype patterns for the QTL cluster regions in weedy and wild rice lines (Table S1). Known genes qsh1, sd1, sh4, Rc, and SD7-1 were positioned on the map according to their physical positions on the rice reference genome sequence (Gramene 2012).

QTL associated with wild and crop-mimic traits

A total of 21 QTL were associated with the six wild traits in the subpopulation, including one for LSC, three for PC, three for HC, six for AN, three for SH, and five for SD (Table 3, see Figure S2, A−F). The number of QTL for a trait varied with the measurements. For example, only one QTL for PC (qPC7, R2 = 0.59) or HC (qHC4, R2 = 0.69) was detected when plants were scored as the presence (1) or absence (0) of the pigments. qPC7 was confirmed and additional two loci (R2 < 0.09) were detected for PC when it was scored as red (2), brown (1), and white (0). Similarly, qHC4 was confirmed and additional two HC loci (R2 <0.09) were detected when the trait was quantified with the reflectance spectra (L*, a*, and b* values). Likewise, three awn loci (i.e., qAN4, 6.1, and 8) were associated with both awn length and percentage of awned seeds and the remaining three QTL (i.e., qAN2, 3, and 6.2) associated with only one of the two measurements. The parent EM93-1 contributed alleles increasing seed dormancy for qSD1.2, black pigment for qHC8, red pigment for qPC6, and awn length or awned-seed for qAN6.1, whereas the parent US1 contributed the effect-increasing alleles to the remaining 17 QTL. Approximately one half of the 21 QTL had a strong dominance effect (d) on PC (qPC6 and 7), HC (qHC1, 4 and 8), AN (qAN6.2), SH (qSH7), or SD (qSD4 and 7.2), as suggested by the d/a ratios of ≥1 or ≤ -1 (Table 3).

View this table:
  • View inline
  • View popup
Table 3 List of QTL associated with wild traits in the F2 EM93-1/US1 population

A total of 15 QTL were associated with the six crop-mimic traits, including three for FT, one for RTN, four for PH, two for SN, two for SW, and three for SSP, in the subpopulation (Table 4, see Figure S2, G−K). The QTL qPH1 (R2 = 0.38) and qFT7 (R2 = 0.13) had a relatively large effect on PH or FT, and the remaining 13 loci contributed <9% to the total phenotypic variances. The US1- and EM93-1−derived alleles increased additive effects for 12 and three of the 15 QTL, respectively. Of the 15 QTL, six displayed a strong dominance effect on FT, RTN, SW, or SSP (Table 4).

View this table:
  • View inline
  • View popup
Table 4 List of QTLs associated with crop-mimic traits expressed during the reproductive growth phase in the F2 EM93-1/US1 population

Six QTL (qPHv) were associated with plant height at weeks 4, 6, or 8 of the vegetative growth phase (Table 5, see Figure S2, L and M). The qPHv1 and qPHv8 QTL were mapped to the same positions as qPH1 and qPH8, respectively (refer to peak positions and nearest markers in Table 4 and Table 5), suggesting that qPHv1 and qPH1 and qPHv8 and qPH8 are underlain by same genes expressed in both of the vegetative and reproductive growth phases. The remaining four qPHv QTL appeared to express only in given period(s) of the vegetative growth phase. Four QTL were associated with plant height-increasing rate (PHR) and three (qPHR1, 2, and 6.1) of the four collocated with the qPHv loci (qPHv1, 2, and 6). The other PHR QTL (qPHR6.2) appeared to express only from weeks 4 to 6. US1 and EM93-1 contributed the effect-increasing alleles to the qPHR1 and 6.1 and the qPHR2 and 6.2 loci, respectively.

View this table:
  • View inline
  • View popup
Table 5 List of QTL associated with crop-mimic traits expressed during the vegetative growth phase in the F2 EM93-1/US1 population

One and two QTL were associated with tiller numbers (qTNv1) and tiller number-increasing rate (qTNR1 and 5), respectively, during the vegetative growth phase (Table 5, see Figure S2N). Both qTNv1 and qTNR1 were same for the peak position and had the effect-increasing allele from EM93-1. qTNR5 (R2 = 0.04) contributed less to the phenotypic variance than qTNR1 (R2 = 0.14) and had the effect-increasing allele from US1.

The aforementioned 49 QTL detected in the subpopulation were all confirmed with the full population, as shown by significant levels (P values) from the SMA for the nearest markers (Table 3, Table 4, and Table 5). In general, SMA underestimated the QTL effects (as shown by R2 values), mainly because of distances between the marker and QTL peak positions. These 49 QTL were located on 10 chromosomes (1 to 9 and 12), with 45 (92%) of them collocated as clusters on 14 chromosomal regions (Figure 3). Of the 14 QTL clusters (CL), one encompasses the QTL for crop-mimic (CL5), two contain the QTL for wild traits (CL4.2 and CL7.2), and the remaining 11 have QTL for both wild and crop mimic traits.

Haplotype variants for QTL cluster regions in U.S. weedy rice

The physical length of haplotypes for the 14 QTL clusters varies from 3.2 to 12.0 Mb. The number of haplotypes varied with the clusters from two to 10 in the sample of 28 U.S. weedy rice lines and from four to 10 in the sample of 14 wild rice lines (Table 6). The correlation between the lengths and haplotype numbers was not significant in the weedy (r = 0.38, P = 0.18) or wild (r = 0.03, P = 0.94) rice samples, suggesting that the physical size was not a major contributor to the haplotypic variation. On average, the number of haplotypes was significantly smaller (t = -3.4, P = 0.002) in the weedy (6.1 ± 2.4) than in the wild (7.5 ± 1.8) rice sample, indicating that U.S. weedy rice is less diverse than wild rice for the QTL-rich regions.

View this table:
  • View inline
  • View popup
Table 6 Summary of haplotypes for the 14 QTL-cluster regions in U.S. weedy rice and wild rice

Shared haplotypes between the weedy and wild rice samples ranged from 10 to 100%, with the average being 52%, across the 14 cluster regions (Table 6). The EM93-1−like haplotypes were present in 10 of the 14 QTL regions and accounted for 30% of the haplotypes on average in the weedy rice sample. In contrast, the EM93-1−like haplotypes were present only in six of the 14 regions and represent 7% of the haplotypes in the wild rice sample. Of the four QTL cluster regions (CL1.1, 1.2, 5, and 7.1) absent of the EM93-1-like haplotypes, CL1.1 and CL1.2 link on an interval of ~40 cM containing sd1 and CL7.1 contains Rc (Figure 3). The absence of the EM93-1−like haplotypes indicates that U.S. red rice populations have maintained relatively large blocks of linkage disequilibrium (LD) to cover the wild-type alleles Sd1 and Rc since the founder genotypes were introduced into the new continent. The US1-like haplotypes were present in all the 14 QTL regions and account for 22% of the haplotypes in the sample of U.S. weedy rice. In contrast, the US1-like haplotypes were present in six of the 14 QTL regions and represent 11% of the haplotypes in the wild rice sample.

Based on the haplotype data for the 14 QTL cluster regions, the 28 weedy rice lines were separated from most (9/14) of the wild rice lines and 26 of the 28 clustered into two groups (Figure 4). Group I includes 10 (nine BHA and one FHA) weedy and 3 BHA wild rice lines, while Group II consists of 16 (15 SH and 1 FHA) weedy rice. The other two red rice lines, R01 (PI-506229, SHA) from California and R09 (PI-653420, FHA) from Louisiana, appeared to be close to FHA or SHA wild rice.

Figure 4
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 4

Phylogenetic relationship of selected U.S. weedy and wild rice lines. The dendrogram is developed based on the haplotypic data for the 14 QTL cluster regions (Figure 3). The weedy red (US1 and R01-27) and wild (W01-14) rice lines belong to the four seed morphological types (Figure S1): black-hull awned (BHA), furrow-hull awned (FHA), straw-hull awned (SHA), and straw-hull anwless (SH).

Discussion

Trait correlations and QTL clusters

This research confirmed the previous observation that wild traits are interrelated phenotypically (Gu et al. 2005c) and also revealed that the phenotypic correlation is common between wild and crop-mimic traits in weedy rice. For example, the characters seed shattering, awn presence, red/brown pericarp color, purple leaf sheath color, and seed dormancy were positively or negatively correlated with several of the eight crop mimic traits in the large F2 population (Table 2). Natural selection tended to assemble the wild characters, also known as “adaptive syndromes,” to make a weed plant weedy (Harlan 1965; Oka 1988). The natural selection in agro-ecosystems is expected, to some extent, to impact weed phenotypes for multiple crop-mimic traits, including plant height- and tiller number-increasing rates that contribute to early plant competitiveness. The plant height and tiller number traits differed in dynamic pattern during the vegetative growth phase (Figure 2, A and D) and were correlated with the final plant height and number of reproductive tillers (Table 2). However, the correlations accounted for only <10% of the phenotypic variances, suggesting that there are additional genes imparting the early plant competitiveness. QTL analysis in the previous and this research provided valuable insight into genetic basis and genomic structures underlying individual wild and crop-mimic traits and trait correlations.

More QTL were detected for wild and crop-mimic traits in this than in the reported research on weedy rice (Bres-Patry et al. 2001; Gu et al. 2005a; Jing et al. 2008; Subudhi et al. 2012; Thurber et al. 2013). In addition to the difference in the parental combination of mapping population from the previous research, a larger population size, the greenhouse condition, and more trait measurements used in this research must have also contributed to the QTL detection. It was unforeseen that greater than 90% of the QTL collocated as clusters on 14 genomic regions. The QTL distribution patterns on the genome explain the observed trait correlations. In addition, more QTL clusters detected in this research indicate that “a small number of chromosomal segments” (Bres-Patry et al. 2001) is insufficient to explain the conspecific weed-crop differentiation for adaptive or domestication-related traits.

Both correlated and uncorrelated traits may have their QTL collocated on the same genomic segments. It was estimated that correlated traits had more collocated QTL or shared QTL clusters (33% on average) than uncorrelated traits (11%) (Gardner and Latta 2007). Of the 49 pairs of trait correlation listed in Table 2, 22 (45%) had one or more shared QTL clusters, which is higher than the previous estimation. On the other hand, of the 70 QTL pairs in the 14 clusters, 45 (64%) displayed trait correlations (29 positive and 16 negative), and the remaining 21 (36%) did not correlate phenotypically in the mapping population (see Table S3). For example, qSD1.2 was clustered with qSN1, qPH1, qPHR1, and qTNR1 in cluster CL1.2, although SD was not correlated with any of the SN, PH, PHR, and TNR traits. A higher level of the correspondence between trait correlations and QTL clusters was observed for the 5 wild traits (SH, SD, AN, HC, and PC) in our previous research, most likely because their heritability levels were relatively high (Gu et al. 2005a). Genetic background effects also interfere with the expression of trait correlation or clustered QTL. For example, the correlation between seed dormancy and plant height and their QTL clusters qSD1.2/qPH1 and qSD7.2/qPH7 were detected only in advanced backcross populations (Ye et al. 2013). Additional data can be found at Table S4, Table S5, and Table S6.

There is evidence that trait correlations in weedy rice could arise from pleiotropic effects of single genes, or the linkage between genes for the correlated traits, or both pleiotropy and linkage, although these two mechanisms are often confounded at the QTL level. Cluster CL7.1 consists of qPC7 for pericarp color, qSD7.1 for seed dormancy, and qFT7 for flowering time. Both qPC7 and qSD7.1 are underlain by Rc and share the same functional nucleotide polymorphism or FNP (Gu et al. 2011), whereas qFT7 has been finely mapped to the position a few centimorgan (~6 Mb) from Rc and genetically isolated from qSD7.1 (Gu and Foley 2007 and unpublished data). Cluster CL1.2 encompasses eight QTL for plant height, seed dormancy, and other growth- related traits and also the known gene sd1 encoding gibberellin 20-oxidase. Fine mapping, gene expression analysis, and gibberellin induction analysis indicated that weedy rice carries the wild allele at sd1 to promote seedling/plant growth and also suggested that the mutant allele sd1 from the parent EM93-1 most likely has a pleiotropic effect on enhanced seed dormancy (Ye et al. 2013). Recent research identified additional FNPs at sd1 (Asano et al. 2011) and new plant height QTL (Kovi et al. 2011) in the CL1.2 region, suggesting that a QTL cluster could be underlain by multiple molecular genetic mechanisms.

Implications of haplotypic variants for the multi-QTL regions

The haplotype analysis revealed that U.S. weedy red rice populations are highly differentiated in genomic regions containing multiple QTL responsible for adaptation and competitiveness. The haplotype diversity suggests that the wild and crop-mimic traits segregating in the mapping population may also differentiate among ecotypes or populations of weedy rice. About 50% of the haplotypes in the 28 weedy rice lines were also present in the 14 lines of the AA-genome wild rice (O. spp.). These shared wild-like haplotypes were presumably derived from introduced founder genotypes, as there was no wild rice in North America, and have been maintained by LD and natural selection during the evolution of red rice populations. Some of the LD blocks are >10 Mb in length (e.g., CL2 and 4.1) or 100% wild-like (e.g., CL1.1, 6.1, and 6.2). The parental line EM93-1 was developed by a hybridization of two semi-dwarf indica cultivars from China (Gu et al. 2004b), which have no direct pedigree relationship with the U.S. weedy rice lines. The presence of EM93-1−like haplotypes in the weedy rice sample could not be a random event, for the frequency (30%) is much higher than that (7%) in the wild rice sample. Therefore, the haplotypes other than the wild-like ones in U.S. red rice populations were most likely incorporated from old cultivars. The haplotypes like the weedy rice parent US1 accounted for ∼20% of the haplotypes in the 28 red rice lines. This is indicative that the current U.S. weedy rice populations may originate from multiple founder genotypes. Further research could extend the haplotype analysis to old cultivars/landraces, including those from aus and indica ecotypes (Reagon et al. 2010), to help identify the founder genotypes of U.S. weedy rice.

U.S. weedy rice populations are often classified based on HC and AN presence/absence in practice. This simple classification system has been shown in a good agreement with the classification based on genomic patterns of randomly selected DNA markers (Reagon et al. 2010) and the haplotype patterns for the QTL cluster regions (Figure 4). This research identified three HC and six AN QTL and these nine loci distribute on nine different genomic segments of six chromosomes (Figure 3). Thurber et al. (2013) detected two HC and three AN QTL from U.S. weedy rice, with two of the AN loci on the two additional chromosomes. Thus, phenotypic variation for the HC and AN morphologies is regulated by at least 11 functionally differentiated loci scattered on eight chromosomes. The genome distribution pattern of the multiple QTL explains the good agreement between the morphological and marker-based classification systems. It is expected that the morphological classification system will continue to be useful because of the genome distribution of HC and AN loci and their linkage with genes for the other adaptive traits in the clusters.

Acknowledgment

Funding for this research was supported in part by grants to X.-Y.G. from United States Department of Agriculture-National Research Initiative (#2008-35301-19058), National Science Foundation (IOS no. 0641376 and no. 1021382), and South Dakota Agricultural Extension Station and Drought Center.

Footnotes

  • Supporting information is available online at http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.113.006395/-/DC1.

  • Communicating editor: S. A. Jackson

  • Received March 14, 2013.
  • Accepted April 15, 2013.
  • Copyright © 2013 Mispan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Literature Cited

  1. ↵
    1. Asano K.,
    2. Yamasaki M.,
    3. Takuno S.,
    4. Katagiri S.,
    5. Ito T.,
    6. et al.
    , 2011 Artificial selection for a green revolution gene during japonica rice domestication. Proc. Natl. Acad. Sci. USA 108: 11034–11039.
    OpenUrlAbstract/FREE Full Text
  2. ↵
    1. Baker H. G.
    , 1974 The evolution of weeds. Annu. Rev. Ecol. Syst. 5: 1–24.
    OpenUrlCrossRef
  3. ↵
    1. Booth B. D.,
    2. Murphy S. D.,
    3. Swanton C. J.
    , 2003 Weed Ecology in Natural and Agricultural Systems. CABI, Wallingford, UK.
  4. ↵
    1. Bres-Patry C.,
    2. Lorieux M.,
    3. Clement G.,
    4. Bangratz M.,
    5. Ghesquiere A.
    , 2001 Heredity and genetic mapping of domestication-related traits in a temperate japonica weedy rice. Theor. Appl. Genet. 102: 118–126.
    OpenUrlCrossRefWeb of Science
  5. ↵
    1. Cao Q.,
    2. Lu B. R.,
    3. Hui X.,
    4. Jun R.,
    5. Sala F.,
    6. et al.
    , 2006 Genetic diversity and origin of weedy rice (Oryza sativa f. spontanea) populations found in north-eastern China revealed by simple sequence repeat (SSR) markers. Ann. Bot. (Lond.) 98: 1241–1252.
    OpenUrlAbstract/FREE Full Text
  6. ↵
    1. Delouche J. C.,
    2. Burgos N. R.,
    3. Gealy D. R.,
    4. de San Martin G. Z.,
    5. Labrada R.
    , 2007 Weedy Rices- Origin, Biology, Ecology and Control. FAO, Rome, Italy.
  7. ↵
    1. Ellstrand N. C.,
    2. Prentice H. C.,
    3. Hancock J. F.
    , 1999 Gene flow and introgression from domesticated plants into their wild relatives. Annu. Rev. Ecol. Syst. 30: 539–563.
    OpenUrlCrossRefWeb of Science
  8. ↵
    1. Furukawa T.,
    2. Maekawa M.,
    3. Oki T.,
    4. Suda I.,
    5. Iida S.,
    6. et al.
    , 2007 The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp. Plant J. 49: 91–102.
    OpenUrlCrossRefPubMedWeb of Science
  9. ↵
    1. Gardner K. M.,
    2. Latta R. G.
    , 2007 Shared quantitative traits loci underlying the genetic correlation between continuous traits. Mol. Ecol. 16: 4195–4209.
    OpenUrlCrossRefPubMedWeb of Science
  10. ↵
    Gramene, 2012 Oryza sativa genome sequence (Gramene release 36). Available at: 2012 at http://www.gramene.org/Oryza_sativa/Info/Index. Accessed: December 2012.
  11. ↵
    1. Gu X.-Y.,
    2. Kianian S. F.,
    3. Foley M. E.
    , 2004a Multiple loci and epistases control genetic variation for seed dormancy in weedy rice (Oryza sativa). Genetics 166: 1503–1516.
    OpenUrlAbstract/FREE Full Text
  12. ↵
    1. Gu X.-Y.,
    2. Foley M. E.,
    3. Chen Z.
    , 2004b A set of three genes regulates photoperiodic responses of flowering in rice (Oryza sativa). Genetica 122: 127–140.
    OpenUrlCrossRefPubMed
  13. ↵
    1. Gu X.-Y.,
    2. Kianian S. F.,
    3. Hareland G. A.,
    4. Hoffer B. L.,
    5. Foley M. E.
    , 2005a Genetic analysis of adaptive syndromes interrelated with seed dormancy in weedy rice (Oryza sativa). Theor. Appl. Genet. 110: 1108–1118.
    OpenUrlCrossRefPubMedWeb of Science
  14. ↵
    1. Gu X.-Y.,
    2. Kianian S. F.,
    3. Foley M. E.
    , 2005b Phenotypic selection for dormancy introduced a set of adaptive haplotypes from weedy into cultivated rice. Genetics 171: 695–704.
    OpenUrlAbstract/FREE Full Text
  15. ↵
    1. Gu X.-Y.,
    2. Kianian S. F.,
    3. Foley M. E.
    , 2005c Dormancy imposed by covering tissues interrelated with seed shattering and morphological characteristics in weedy rice (Oryza sativa L.). Crop Sci. 45: 948–955.
    OpenUrlCrossRef
  16. ↵
    1. Gu X.-Y.,
    2. Foley M. E.
    , 2007 Epistatic interactions of three loci regulate flowering time under short and long daylengths in a backcross population of rice. Theor. Appl. Genet. 114: 745–754.
    OpenUrlCrossRefPubMedWeb of Science
  17. ↵
    1. Gu X.-Y.,
    2. Foley M. E.,
    3. Horvath D. P.,
    4. Anderson J. V.,
    5. Feng J.,
    6. et al.
    , 2011 Association between seed dormancy and pericarp color is controlled by a pleiotropic gene that regulates abscisic acid and flavonoid synthesis in weedy red rice. Genetics 189: 1515–1524.
    OpenUrlAbstract/FREE Full Text
  18. ↵
    1. Harlan J. R.
    , 1965 The possible role of weed races in the evolution of cultivated plants. Euphytica 14: 173–176.
    OpenUrlCrossRefWeb of Science
  19. ↵
    1. Ishikawa R.,
    2. Toki N.,
    3. Imai K.,
    4. Sato Y.,
    5. Yamagishi H.,
    6. et al.
    , 2005 Origin of weedy rice grown in Bhutan and the force of genetic diversity. Genet. Resour. Crop Evol. 52: 395–403.
    OpenUrlCrossRef
  20. ↵
    1. Jing W.,
    2. Jiang L.,
    3. Zhang W.,
    4. Zhai H.,
    5. Wan J.
    , 2008 Mapping QTL for seed dormancy in weedy rice. Acta Agron. Sin. 34: 737–742.
    OpenUrl
  21. ↵
    1. Konishi S.,
    2. Izawa T.,
    3. Lin S. Y.,
    4. Ebana K.,
    5. Fukuta Y.,
    6. et al.
    , 2006 An SNP caused loss of seed shattering during rice domestication. Science 312: 1392–1396.
    OpenUrlAbstract/FREE Full Text
  22. ↵
    1. Kovi M. R.,
    2. Zhang Y.,
    3. Yu S.,
    4. Yang G.,
    5. Yan W.,
    6. et al.
    , 2011 Candidacy of a chitin-inducible gibberellins-responsive gene for a major locus affecting plant height in rice that is closely linked to Green Revolution gene sd1. Theor. Appl. Genet. 123: 705–714.
    OpenUrlCrossRefPubMed
  23. ↵
    1. Chopra V. L.,
    2. Prakash S.
    1. Kush G. S.,
    2. Brar D. S.
    , 2002 Rice, pp. 1–41 in Evolution and Adaptation of Cereal Crops, edited by Chopra V. L., Prakash S.. Science Publishers Inc., Enfield, NH.
  24. ↵
    1. Li C.,
    2. Zhou A.,
    3. Sang T.
    , 2006 Rice domestication by reducing shattering. Science 311: 1936–1939.
    OpenUrlAbstract/FREE Full Text
  25. ↵
    1. Lincoln S.,
    2. Daly M.,
    3. Lander E.
    , 1992 Constructing Genetic Maps with MAPMAKER/EXP 3.0. 3rd ed. Whitehead Institute, Cambridge, MA.
  26. ↵
    1. Londo J. P.,
    2. Schaal B. A.
    , 2007 Origins and population genetics of weedy red rice in the USA. Mol. Ecol. 16: 4523–4535.
    OpenUrlCrossRefPubMedWeb of Science
  27. ↵
    1. McCouch S. R.,
    2. Teytelman L.,
    3. Xu Y. B.,
    4. Lobos K. B.,
    5. Clare K.
    , 2002 Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res. 9: 199–207.
    OpenUrlAbstract
  28. ↵
    1. Noldin J. A.,
    2. Chandler J. M.,
    3. McCauley G. N.
    , 1999 Red rice (Oryza sativa) Biology. I. Characterization of red rice ecotypes. Weed Technol. 13: 12–18.
    OpenUrl
  29. ↵
    1. Oka H. I.
    , 1988 Origin of Cultivated Rice. Japan Science Society Press, Tokyo.
  30. ↵
    1. Reagon M.,
    2. Thurber C. S.,
    3. Gross B. L.,
    4. Olsen K. M.,
    5. Jia Y.,
    6. et al.
    , 2010 Genomic patterns of nucleotide diversity in parallel populations of U.S. weedy rice. BMC Evol. Biol. 10: 180.
    OpenUrlCrossRefPubMed
  31. ↵
    1. Reagon M.,
    2. Thurber C. S.,
    3. Olsen K. M.,
    4. Jia Y.,
    5. Caicedo A. L.
    , 2011 The long and the short of it: SD1 polymorphism and the evolution of growth trait divergence in U.S. weedy rice. Mol. Ecol. 20: 3743–3756.
    OpenUrlCrossRefPubMedWeb of Science
  32. ↵
    1. SAS Institute
    , 2011 SAS/STAT 9.3 User’s Guide. SAS Institute Inc, Cary, NC.
  33. ↵
    1. Subudhi P. K.,
    2. Parco A.,
    3. Singh P. K.,
    4. DeLeon T.,
    5. Karan R.,
    6. et al.
    , 2012 Genetic architecture of seed dormancy in U.S. weedy rice in different genetics backgrounds. Crop Sci. 52: 2564–2575.
    OpenUrlCrossRefWeb of Science
  34. ↵
    1. Suh H. S.,
    2. Sato Y. I.,
    3. Morishima H.
    , 1997 Genetic characterization of weedy rice (Oryza sativa L.) based on morphophysiology, isozymes and RAPD markers. Theor. Appl. Genet. 94: 316–321.
    OpenUrlCrossRef
  35. ↵
    1. Sweeney M. T.,
    2. Thomson M. J.,
    3. Pfeil B. E.,
    4. McCouch S. R.
    , 2006 Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice. Plant Cell 18: 283–294.
    OpenUrlAbstract/FREE Full Text
  36. ↵
    1. Tang L. H.,
    2. Morishima H.
    , 1997 Genetic characterization of weedy rices and the inference on their origins. Breed. Sci. 47: 153–160.
    OpenUrl
  37. ↵
    1. Thurber C. S.,
    2. Reagon M.,
    3. Gross B. L.,
    4. Olsen K. M.,
    5. Jia Y.,
    6. et al.
    , 2010 Molecular evolution of shattering loci in U.S. weedy rice. Mol. Ecol. 19: 3271–3284.
    OpenUrlCrossRefPubMedWeb of Science
  38. ↵
    1. Thurber C. S.,
    2. Jia M. H.,
    3. Jia Y.,
    4. Caicedo A. L.
    , 2013 Similar traits, different genes? Examining convergent evolution in related weedy rice populations. Mol. Ecol. 22: 685–698.
    OpenUrlCrossRefWeb of Science
  39. ↵
    Wang, S., C. J. Basten, and Z. B. Zeng, 2006 QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, http://statgen.ncsu.edu/qtlcart/WQTLCart.htm
  40. ↵
    1. Ye H.,
    2. Foley M. E.,
    3. Gu X.-Y.
    , 2010 New seed dormancy loci detected from weedy rice-derived advanced populations with major QTL alleles removed from the background. Plant Sci. 179: 612–619.
    OpenUrlCrossRef
  41. ↵
    1. Ye H.,
    2. Beighley D. H.,
    3. Feng J.,
    4. Gu X.-Y.
    , 2013 Genetic and physiological characterization of two clusters of quantitative trait loci associated with seed dormancy and plant height in rice. G3 (Bethesda) 3: 323–331.
    OpenUrlAbstract/FREE Full Text
  42. ↵
    1. Yoshida S.,
    2. Forno D. A.,
    3. Cock J. H.,
    4. Gomez K. A.
    , 1976 Laboratory Manual For Physiological Studies Of Rice. 3rd ed. IRRI, Manila, The Philippines.
View Abstract
Previous ArticleNext Article
Back to top

PUBLICATION INFORMATION

Volume 3 Issue 6, June 2013

G3: Genes|Genomes|Genetics: 3 (6)

ARTICLE CLASSIFICATION

Investigations
View this article with LENS
Email

Thank you for sharing this G3: Genes | Genomes | Genetics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Quantitative Trait Locus and Haplotype Analyses of Wild and Crop-Mimic Traits in U.S. Weedy Rice
(Your Name) has forwarded a page to you from G3: Genes | Genomes | Genetics
(Your Name) thought you would be interested in this article in G3: Genes | Genomes | Genetics.
Print
Alerts
Enter your email below to set up alert notifications for new article, or to manage your existing alerts.
SIGN UP OR SIGN IN WITH YOUR EMAIL
View PDF
Share

Quantitative Trait Locus and Haplotype Analyses of Wild and Crop-Mimic Traits in U.S. Weedy Rice

Muhamad S. Mispan, Lihua Zhang, Jiuhuan Feng and Xing-You Gu
G3: Genes, Genomes, Genetics June 1, 2013 vol. 3 no. 6 1049-1059; https://doi.org/10.1534/g3.113.006395
Muhamad S. Mispan
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lihua Zhang
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiuhuan Feng
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xing-You Gu
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Xingyou.gu@sdstate.edu
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation

Quantitative Trait Locus and Haplotype Analyses of Wild and Crop-Mimic Traits in U.S. Weedy Rice

Muhamad S. Mispan, Lihua Zhang, Jiuhuan Feng and Xing-You Gu
G3: Genes, Genomes, Genetics June 1, 2013 vol. 3 no. 6 1049-1059; https://doi.org/10.1534/g3.113.006395
Muhamad S. Mispan
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lihua Zhang
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiuhuan Feng
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xing-You Gu
Plant Science Department, South Dakota State University, Brookings, South Dakota 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Xingyou.gu@sdstate.edu

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Related Articles

Cited By

More in this TOC Section

  • Photosensitive Alternative Splicing of the Circadian Clock Gene timeless Is Population Specific in a Cold-Adapted Fly, Drosophila montana
  • Maize Transposable Elements Ac/Ds as Insertion Mutagenesis Tools in Candida albicans
  • N-terminal Sumoylation of Centromeric Histone H3 Variant Cse4 Regulates Its Proteolysis To Prevent Mislocalization to Non-centromeric Chromatin
Show more Investigations
  • Top
  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgment
    • Footnotes
    • Literature Cited
  • Figures & Data
  • Supplemental
  • Info & Metrics

GSA

The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.

Online ISSN: 2160-1836

  • For Authors
  • For Reviewers
  • For Advertisers
  • Submit a Manuscript
  • Editorial Board
  • Press Releases

SPPA Logo

GET CONNECTED

RSS  Subscribe with RSS.

email  Subscribe via email. Sign up to receive alert notifications of new articles.

  • Facebook
  • Twitter
  • YouTube
  • LinkedIn
  • Google Plus

Copyright © 2018 by the Genetics Society of America

  • About G3
  • Terms of use
  • Permissions
  • Contact us
  • International access